Wu P, Brockenbrough J S, Paddy M R, Aris J P
Department of Anatomy and Cell Biology, Health Science Center, Box 100235, University of Florida, Gainesville, FL 32610, USA.
Gene. 1998 Oct 5;220(1-2):109-17. doi: 10.1016/s0378-1119(98)00330-8.
The nucleolar protein Nop2p is an essential gene product that is required for pre-rRNA processing and ribosome biogenesis in Saccharomyces cerevisiae (Hong, B. et al., 1997, Mol. Cell. Biol., 17, 378-388). A search for proteins similar to Nop2p identified a novel yeast gene product that also shares significant homology with the human proliferation associated nucleolar protein p120. The gene encoding this 78kDa protein was termed NCL1 (for nuclear protein 1; corresponding to YBL024w). Ncl1p and Nop2p contain an evolutionarily conserved motif that has been termed the 'NOL1/NOP2/fmu family signature' (NOL1 encodes p120). Epitope tagged Ncl1p was found to be localized to the nucleus, including the nucleolus, and was concentrated at the nuclear periphery. NCL1 is not essential. Strains containing a disruption of NCL1, or strains overexpressing NCL1, grow essentially identically to wildtype NCL1 strains on a number of different media and at different temperatures. Disruption of NCL1 does not affect steady-state levels of large and small ribosome subunits, monoribosomes, and polyribosomes. However, disruption of NCL1 leads to increased sensitivity to the antibiotic paromomycin.
核仁蛋白Nop2p是酿酒酵母中前体rRNA加工和核糖体生物合成所必需的一种重要基因产物（Hong, B.等人，1997年，《分子与细胞生物学》，17卷，378 - 388页）。对与Nop2p相似的蛋白质进行搜索，鉴定出一种新的酵母基因产物，它与人增殖相关核仁蛋白p120也有显著的同源性。编码这种78kDa蛋白质的基因被命名为NCL1（核蛋白1；对应于YBL024w）。Ncl1p和Nop2p含有一个进化上保守的基序，被称为“NOL1/NOP2/fmu家族特征序列”（NOL1编码p120）。发现表位标记的Ncl1p定位于细胞核，包括核仁，并集中在核周边。NCL1不是必需基因。含有NCL1缺失的菌株，或过表达NCL1的菌株，在多种不同培养基上以及不同温度下，其生长情况与野生型NCL1菌株基本相同。NCL1的缺失不影响大、小核糖体亚基、单核糖体和多核糖体的稳态水平。然而，NCL1的缺失会导致对抗生素巴龙霉素的敏感性增加。
