Tanaka N, Tajima S, Ishibashi A, Izumi T, Nishina S, Azuma N, Sado Y, Ninomiya Y
Department of Dermatology, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359, Japan.
Br J Dermatol. 1998 Sep;139(3):371-4. doi: 10.1046/j.1365-2133.1998.02397.x.
To study the expression of the alpha1-alpha6 chains of type IV collagen in the dermoepidermal junction (DEJ) during human foetal skin development, human foetal (10 and 20 weeks of gestation) and adult skin was immunostained with specific monoclonal antibodies to the alpha1-alpha6 chains of type IV collagen. Intense expression of the alpha4 chain and weak expression of the alpha2 and alpha6 chains were observed in the DEJ of 10 weeks gestational skin. In contrast, the alpha1, alpha2, alpha5 and alpha6 chains were detected in the DEJ of 20 weeks gestational and adult skin. Preferential expression of alpha4 during the early phase of development (10 weeks of gestation) may suggest a chain-specific regulatory mechanism for type IV collagen expression and its potential role in DEJ formation during development.
为研究人胎儿皮肤发育过程中Ⅳ型胶原α1-α6链在真皮表皮连接处（DEJ）的表达情况，用针对Ⅳ型胶原α1-α6链的特异性单克隆抗体对人胎儿（妊娠10周和20周）及成人皮肤进行免疫染色。在妊娠10周皮肤的DEJ中观察到α4链的强烈表达以及α2和α6链的弱表达。相比之下，在妊娠20周及成人皮肤的DEJ中检测到α1、α2、α5和α6链。发育早期（妊娠10周）α4链的优先表达可能提示Ⅳ型胶原表达存在链特异性调控机制及其在发育过程中对DEJ形成的潜在作用。
