Bíró L, Domján G, Falus A, Jakab L, Cseh K, Kalabay L, Tarkovács G, Tresch J, Malle E, Kramer J, Prohászka Z, Jákó J, Füst G, Császár A
National Institute of Haematology and Immunology, Budapest, Hungary.
Eur J Clin Invest. 1998 Aug;28(8):679-86. doi: 10.1046/j.1365-2362.1998.00333.x.
Interleukin (IL) 6 has an important role in the regulation of acute-phase proteins (APPs) during an acute-phase response. We studied IL-6 and other cytokines to determine if they regulate serum APP levels in the same way under the condition of the aberrant, long-lasting 'acute-phase response' that occurs in patients with chronic inflammation and cancer.
Serum levels of nine positive APPs [CRP, SAA, C1-INH, Bf, C5, C8, C9, alpha 1-acidic glycoprotein (AGP) and haptoglobin] and two negative APPs [transferrin and alpha 2-HS glycoprotein (AHSG)] were measured using immunochemical methods in 59 multiple myeloma patients and in 72 healthy control subjects. Serum IL-6 and tumour necrosis factor (TNF) alpha levels were determined by bioassays.
IL-6 was negatively correlated with five out of nine (C1-INH, C8, C9, AGP and haptoglobin) positive APPs but positively correlated with C-reactive protein (CRP). When patients with high and low IL-6 serum concentration were compared, CRP levels were higher, AGP and haptoglobin levels were lower in the high- than in the low-L-6 group, whereas no significant difference between the two groups was found in levels of the other positive and negative APPs. TNF-alpha levels were negatively correlated with transferrin and AHSG levels. No difference in the levels of positive APPs was observed between patients with low and high TNF-alpha serum concentration. By contrast, levels of both transferrin and AHSG were significantly lower in the high- than in the low-TNF-alpha group.
These findings indicate that, except for regulation of the negative APPs by TNF-alpha, the mechanism of APP regulation is different under the conditions of the short-term and the chronic, long-lasting 'acute-phase reaction'.
白细胞介素（IL）6在急性期反应期间对急性期蛋白（APPs）的调节中起重要作用。我们研究了IL-6和其他细胞因子，以确定在慢性炎症和癌症患者中出现的异常、持久的“急性期反应”情况下，它们是否以相同方式调节血清APP水平。
采用免疫化学方法检测了59例多发性骨髓瘤患者和72例健康对照者血清中9种阳性APPs[CRP、SAA、C1-INH、Bf、C5、C8、C9、α1-酸性糖蛋白（AGP）和触珠蛋白]和2种阴性APPs[转铁蛋白和α2-HS糖蛋白（AHSG）]的水平。通过生物测定法测定血清IL-6和肿瘤坏死因子（TNF）α水平。
IL-6与9种阳性APPs中的5种（C1-INH、C8、C9、AGP和触珠蛋白）呈负相关，但与C反应蛋白（CRP）呈正相关。比较IL-6血清浓度高和低的患者时，高IL-6组的CRP水平较高，AGP和触珠蛋白水平较低，而两组之间其他阳性和阴性APPs水平无显著差异。TNF-α水平与转铁蛋白和AHSG水平呈负相关。TNF-α血清浓度低和高的患者之间阳性APPs水平无差异。相比之下，高TNF-α组的转铁蛋白和AHSG水平均显著低于低TNF-α组。
这些发现表明，除了TNF-α对阴性APPs的调节外，在短期和慢性、持久的“急性期反应”条件下，APP调节机制不同。
