Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland.
Department of Epizootiology and Clinic of Infectious Diseases, Faculty of Veterinary Medicine, University of Life Sciences in Lublin, Lublin 20-612, Poland.
Poult Sci. 2020 May;99(5):2424-2437. doi: 10.1016/j.psj.2020.01.003. Epub 2020 Mar 13.
The aim of the this study was to determine the effect of different levels of Transcarpathian zeolite (clinoptilolite) on selected indicators of the immune response in chickens by assessing the concentrations of the acute phase proteins haptoglobin, C-reactive protein (CRP), serum amyloid A, transferrin, and alpha-1-acid glycoprotein and the cytokines tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), IL-2, and IL-10 in the serum and hepatic tissues of chickens. The study was conducted on 450 one-day-old male Ross 308 chickens. The total rearing period was 42 D. The samples of blood and liver were collected before the start of the study (day 0) and on day 42, after rearing was completed. ELISA kits specific for chicken CRP, haptoglobin, alpha-1-acid glycoprotein, serum amyloid A, transferrin, TNF-α, IFN-γ, IL-2, and IL-10 were used to determine the levels of acute phase proteins and cytokines in the serum and liver homogenates. The results of immunological tests suggest that for long-term maintenance of homeostasis in chickens, the addition of 2% zeolite as a feed additive is most beneficial. The results indicate that 3% clinoptilolite induce production of Th1 pro-inflammatory cytokines, increasing the synthesis of IL-2, IFN-γ, and TNF-α. The high concentration of IL-10 after the use of zeolite in conjunction with the high concentration of IL-2, TNF-α, and IFN-γ indicates a reduction in the intensity of inflammatory processes, the enhancement of the humoral immune response, and the simultaneous inhibition of the production of Th1-type cytokines. The increase of CRP concentration in conjunction with high concentrations of pro- and anti-inflammatory cytokines in the birds from the group receiving 3% clinoptilolite demonstrates indicates that it can influence the development of local inflammatory processes and enhance immune regulation in birds. Our research has shown that clinoptilolite influences on an increase in birds' resistance to infection, as confirmed by clinical observations and anatomopathological examination and by the increase in the synthesis of acute phase proteins with immunoregulatory properties.
本研究旨在通过评估急性相蛋白（触珠蛋白、C 反应蛋白 (CRP)、血清淀粉样蛋白 A、转铁蛋白和α-1-酸性糖蛋白）和细胞因子（肿瘤坏死因子-α (TNF-α)、干扰素-γ (IFN-γ)、IL-2 和 IL-10）在血清和肝脏组织中的浓度，来确定不同水平的特卡波西沸石（斜发沸石）对鸡免疫反应的选择指标的影响。研究对象为 450 只 1 日龄雄性罗斯 308 鸡。总饲养期为 42 天。在研究开始前（第 0 天）和饲养结束后的第 42 天采集血液和肝脏样本。使用针对鸡 CRP、触珠蛋白、α-1-酸性糖蛋白、血清淀粉样蛋白 A、转铁蛋白、TNF-α、IFN-γ、IL-2 和 IL-10 的 ELISA 试剂盒来确定血清和肝匀浆中急性相蛋白和细胞因子的水平。免疫测试结果表明，对于鸡的长期维持体内平衡，添加 2%沸石作为饲料添加剂最有益。结果表明，3%斜发沸石诱导产生 Th1 促炎细胞因子，增加 IL-2、IFN-γ 和 TNF-α 的合成。使用沸石后 IL-10 浓度升高，同时 IL-2、TNF-α 和 IFN-γ 浓度升高，表明炎症过程强度降低，体液免疫反应增强，同时抑制 Th1 型细胞因子的产生。与接受 3%斜发沸石的鸟类中的高浓度 CRP 浓度相结合，高浓度的促炎和抗炎细胞因子表明它可以影响局部炎症过程的发展并增强鸟类的免疫调节。我们的研究表明，斜发沸石会影响鸟类对感染的抵抗力增强，这得到了临床观察、解剖病理学检查以及具有免疫调节特性的急性相蛋白合成增加的证实。
