Wagelie-Steffen A L, Hartmann K, Vliagoftis H, Metcalfe D D
Laboratory of Allergic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892-1881, USA.
Immunology. 1998 Aug;94(4):569-74. doi: 10.1046/j.1365-2567.1998.00559.x.
Fas ligand (FasL, CD95L, Apo-1L), a type II membrane protein belonging to the tumour necrosis factor family, induces apoptosis in Fas-bearing cells. As murine mast cells have been shown to express Fas antigen, we hypothesized that mast cells might also express FasL. To explore this possibility, we first demonstrated FasL mRNA in mast cells by reverse transcription-polymerase chain reaction and FasL protein by immunoblot analysis. FasL protein was shown to be exclusively located within the cell by flow cytometry. In agreement with this observation, bone marrow cultured mast cells were unable to kill Jurkat T cells. Our results demonstrate that FasL is expressed in murine mast cells and suggest that this murine mast cell FasL is not lytic, owing to the intracellular localization.
Fas配体（FasL、CD95L、Apo-1L）是一种属于肿瘤坏死因子家族的II型膜蛋白，可诱导表达Fas的细胞发生凋亡。由于已证明小鼠肥大细胞表达Fas抗原，我们推测肥大细胞可能也表达FasL。为探究这种可能性，我们首先通过逆转录-聚合酶链反应在肥大细胞中证实了FasL mRNA的存在，并通过免疫印迹分析证实了FasL蛋白的存在。流式细胞术显示FasL蛋白仅位于细胞内。与这一观察结果一致，骨髓培养的肥大细胞无法杀伤Jurkat T细胞。我们的结果表明FasL在小鼠肥大细胞中表达，并且提示由于其细胞内定位，这种小鼠肥大细胞FasL没有细胞溶解作用。
