González Garriga M, García Sánchez H M, Hermida Díaz C, Marcet Sánchez R, Monté Boada R
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1992;44(3):185-7.
The obtention of two monoclonal antibodies which recognize a single epitope present in the subunit B of the thermolabile toxin of Vibrio cholerae and another which shows a cross-reaction with those produced by certain enteropathogenic toxins, is reported. The standardization of a solid phase indirect immunoenzymatic assay (ELISA) for the primary screening and selection of hybrids was performed; in addition, the isotype was determined.
据报道，获得了两种单克隆抗体，它们识别霍乱弧菌不耐热毒素B亚基中存在的单个表位，以及另一种与某些肠道致病毒素产生的抗体发生交叉反应的单克隆抗体。对用于杂交体初步筛选和选择的固相间接免疫酶测定法（ELISA）进行了标准化；此外，还确定了同种型。
