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[在古巴热带条件下大量繁殖库蚊类线虫(线虫纲:索科线虫科)]

[Mass breeding of Romanomermis culicivorax (Nematoda: Mermithidae) in the tropical conditions of Cuba].

作者信息

Santamarina Mijares A

机构信息

Instituto de Medicina Tropical Pedro Kouri.

出版信息

Rev Cubana Med Trop. 1996;48(1):26-33.

PMID:9768266
Abstract

To develop the process of mass production on a large scale of the parasite nematode Romanomermis culicivorax Ross and Smith, 1976, under the control tropical conditions of Cuba, it was taken into consideration the standardization of a certain number of variables, such as: utilization of eggs in the process of infectation, dosage, types of water, types of substrate, temperature, culture methods, and storage methods of culture batches. High performances in nematoda and approximately the same amount of females and males were obtained when mosquito larvae of the species Culex quinquefasciatus Say, 1823, were exposed to parasite's infective larvae.

摘要

为了在古巴的热带条件控制下大规模开展寄生线虫罗氏沼蚊索线虫(Romanomermis culicivorax Ross and Smith,1976)的大规模生产过程,考虑了若干变量的标准化,例如:感染过程中卵的利用、剂量、水的类型、底物类型、温度、培养方法以及培养批次的储存方法。当将1823年的致倦库蚊(Culex quinquefasciatus Say)的蚊幼虫暴露于寄生虫的感染性幼虫时,获得了线虫的高性能以及大致相同数量的雌性和雄性个体。

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