Bello M, Más Lago P, Palomera R, Castillo A, Amín N, Acosta B, Cartaya J, Avalos I
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1996;48(2):118-22.
The results of the study of Enterovirus as viral meningoencephalitis producing agents, carried out from 1990 to 1994, are described, 546 feces samples, 95 cerebrospinal fluids and 1,058 matched sera were studied and obtained from 1,388 patients clinically diagnosed with this disease. Samples for viral isolation were inoculated into two different cellular systems. The highest number of isolation was found in diploid cells from human fibroblast. Antibody determinations were carried out by a neutralization test (micromethod) with 11 Enterovirus antigens (Echo 4, 6, 9, 11 and 30; and Coxsackie B1, 2, 3, 4, 5 and 6) and in epidemic periods with the isolated virus. During the years under study, 2 epidemic outbreaks took place: on caused by Coxsackie A9 (1990-1991) and the other one by Echo 30 (1994). A greater positivity to Echo 6 and 11 was found among the matched sera.
本文描述了1990年至1994年期间开展的关于肠道病毒作为病毒性脑膜脑炎致病原的研究结果。从1388例临床诊断为此病的患者身上采集了546份粪便样本、95份脑脊液样本和1058份配对血清样本进行研究。将用于病毒分离的样本接种到两种不同的细胞系中。在人成纤维细胞的二倍体细胞中分离出的病毒数量最多。采用中和试验（微量法），使用11种肠道病毒抗原（埃可病毒4型、6型、9型、11型和30型；柯萨奇病毒B1型、2型、3型、4型、5型和6型）以及在流行期间使用分离出的病毒进行抗体测定。在研究期间，发生了2次疫情爆发：一次由柯萨奇A9病毒引起（1990 - 1991年），另一次由埃可病毒30型引起（1994年）。在配对血清中发现对埃可病毒6型和11型的阳性反应更高。
