Chacón D, Valdivia A, Goyenechea A, Oropesa I, Savón C
Instituto de Medicina Tropical Pedro Kouri.
Rev Cubana Med Trop. 1996;48(2):136-7.
A high number of acute respiratory diseases was detected among children under one year admitted in a hospital of Havana City. 25 respiratory syncytial virus strains were obtained from 93 patients studied. Viral isolations were multiplied in HEP-2 cells and after observing a cytopathic effect of 80%, they were classified into subgroups by the indirect immunofluorescence technique, using anti-protein G antibodies from the respiratory syncytial virus. All the samples studied were classified within subgroup A. It is the first time a study like this is conducted in our country, which allowed us to deepen into the viral cause of these diseases and to know that the subgroup A of the respiratory syncytial virus circulated during the outbreak.
在哈瓦那市一家医院收治的一岁以下儿童中检测到大量急性呼吸道疾病。从93名研究对象中分离出25株呼吸道合胞病毒。病毒分离株在HEP - 2细胞中增殖，在观察到80%的细胞病变效应后，使用呼吸道合胞病毒的抗蛋白G抗体，通过间接免疫荧光技术将其分为亚组。所有研究样本均被归类为A亚组。这是我国首次开展此类研究，该研究使我们能够深入了解这些疾病的病毒病因，并得知呼吸道合胞病毒A亚组在此次疫情中传播。
