Kitani M, Sakata J, Asada Y, Kitamura K, Eto T
First Department of Internal Medicine, Miyazaki Medical College, Japan.
Ann Clin Biochem. 1998 Sep;35 ( Pt 5):643-8. doi: 10.1177/000456329803500508.
Adrenomedullin (AM) is a biologically active peptide recently isolated from phaeochromocytoma. We report here the distribution and characterization of immunoreactive AM and gene expression of AM in human gastrointestinal tissue. Using a sensitive radioimmunoassay system for the peptide, immunoreactive human AM was detected in the stomach, duodenum, jejunum, ileum and colon. The AM concentration of these tissues was about 0.4-0.8 pmol/g wet tissue. Reverse phase and gel filtration high-performance liquid chromatographies showed that most of the immunoreactive AM in stomach and jejunum was identical to authentic human AM. By northern blot analysis, human AM mRNA was found to be expressed ubiquitously in the human gastrointestinal tissues. Furthermore, an immunohistochemical study revealed that immunoreactive AM cells were present in the gastrointestinal glands. These results suggest that AM may play some role as a gastrointestinal hormone.
肾上腺髓质素（AM）是一种最近从嗜铬细胞瘤中分离出的生物活性肽。我们在此报告免疫反应性AM在人胃肠道组织中的分布、特征以及AM的基因表达情况。使用针对该肽的灵敏放射免疫分析系统，在胃、十二指肠、空肠、回肠和结肠中检测到了免疫反应性人AM。这些组织中的AM浓度约为0.4 - 0.8 pmol/g湿组织。反相和凝胶过滤高效液相色谱显示，胃和空肠中大部分免疫反应性AM与 authentic human AM相同。通过Northern印迹分析，发现人AM mRNA在人胃肠道组织中普遍表达。此外，免疫组织化学研究表明，胃肠道腺体中存在免疫反应性AM细胞。这些结果提示AM可能作为一种胃肠激素发挥某些作用。
