免疫反应性大鼠肾上腺髓质素在组织和血浆中的分布及特性

Distribution and characterization of immunoreactive rat adrenomedullin in tissue and plasma.

作者信息

Sakata J, Shimokubo T, Kitamura K, Nishizono M, Iehiki Y, Kangawa K, Matsuo H, Eto T

机构信息

First Department of Internal Medicine, Miyazaki Medical College, Japan.

出版信息

FEBS Lett. 1994 Sep 26;352(2):105-8. doi: 10.1016/0014-5793(94)00928-7.

DOI:10.1016/0014-5793(94)00928-7

PMID:7925955

Abstract

Adrenomedullin is a new bioactive peptide recently isolated from pheochromocytoma. We report on the rat adrenomedullin distribution and molecular forms in various tissues and plasma. Using a sensitive radioimmunoassay system for rat adrenomedullin, high concentrations of immunoreactive rat adrenomedullin were detected in adrenal gland, lung and cardiac atrium. In lung and atrium, the immunoreactivity concentration in rat was about 6-10 times higher than that in human. The mean plasma concentration of immunoreactive rat adrenomedullin was 3.60 +/- 0.34 fmol/ml (mean +/- S.D.). Analysis in adrenal gland, lung and atrium with reverse-phase and gel-filtration high-performance liquid chromatography showed that most immunoreactive rat adrenomedullin emerged as a single peak at a position exactly identical to that of the authentic rat adrenomedullin peptide, synthesized according to the sequence predicted from the cDNA.

摘要

肾上腺髓质素是一种最近从嗜铬细胞瘤中分离出的新型生物活性肽。我们报告了大鼠肾上腺髓质素在各种组织和血浆中的分布及分子形式。使用针对大鼠肾上腺髓质素的灵敏放射免疫分析系统，在肾上腺、肺和心房中检测到高浓度的免疫反应性大鼠肾上腺髓质素。在肺和心房中，大鼠的免疫反应性浓度比人类高约6至10倍。免疫反应性大鼠肾上腺髓质素的平均血浆浓度为3.60 +/- 0.34 fmol/ml（平均值 +/- 标准差）。用反相和凝胶过滤高效液相色谱法对肾上腺、肺和心房进行分析表明，大多数免疫反应性大鼠肾上腺髓质素在与根据cDNA预测序列合成的 authentic大鼠肾上腺髓质素肽完全相同的位置出现单一峰。