Caldwell R G, Wilson J B, Anderson S J, Longnecker R
Department of Microbiology-Immunology, Northwestern University Medical School, Chicago, Illinois 60611, USA.
Immunity. 1998 Sep;9(3):405-11. doi: 10.1016/s1074-7613(00)80623-8.
Epstein-Barr virus (EBV) establishes a persistent latent infection in peripheral B lymphocytes in humans and is associated with a variety of malignancies and proliferative disorders. Latent membrane protein 2A (LMP2A) is one of only two viral proteins expressed in latently infected B lymphocytes in vivo. LMP2A blocks B cell receptor (BCR) signal transduction in vitro by binding the Syk and Lyn protein tyrosine kinases. To analyze the significance of LMP2A expression in vivo, transgenic mice with B cell lineage expression of LMP2A were generated. LMP2A expression results in the bypass of normal B lymphocyte developmental checkpoints allowing immunoglobulin-negative cells to colonize peripheral lymphoid organs, indicating that LMP2A possesses a constitutive signaling activity in nontransformed cells.
爱泼斯坦-巴尔病毒（EBV）在人类外周B淋巴细胞中建立持续的潜伏感染，并与多种恶性肿瘤和增殖性疾病相关。潜伏膜蛋白2A（LMP2A）是体内潜伏感染的B淋巴细胞中仅有的两种表达的病毒蛋白之一。LMP2A在体外通过结合Syk和Lyn蛋白酪氨酸激酶来阻断B细胞受体（BCR）信号转导。为了分析LMP2A在体内表达的意义，构建了具有B细胞谱系表达LMP2A的转基因小鼠。LMP2A的表达导致正常B淋巴细胞发育检查点的绕过，使免疫球蛋白阴性细胞定殖于外周淋巴器官，这表明LMP2A在未转化细胞中具有组成性信号活性。
