Hey J M, MacFarlane D R
Department of Chemistry, Monash University, Wellington Road, Clayton, Victoria, 3168, Australia.
Cryobiology. 1998 Sep;37(2):119-30. doi: 10.1006/cryo.1998.2108.
The crystallization of ice in aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using a combined DSC-video microscope technique. The solutions investigated were 50w/w% glycerol and 45w/w% Me2SO; both of these solutions have a solute concentration of approximately 16 mol%. The rates of growth of the external surfaces of ice crystals from both of these solutions were determined over broad temperature ranges. The growth rates were found to be generally independent of time, particularly at lower temperatures. The ice crystal growth rate in the glycerol solution became negligible at a significantly higher temperature than in the Me2SO solution. Addition of anti-freeze protein from the winter flounder at concentrations of 1.7 and 9.9 mg g-1 was found to have no significant effect on the ice crystal growth rates in 50w/w% glycerol solutions.
采用差示扫描量热仪-视频显微镜联用技术，研究了甘油和二甲基亚砜（Me2SO）水溶液中冰的结晶过程。所研究的溶液为50w/w%甘油溶液和45w/w%Me2SO溶液；这两种溶液的溶质浓度均约为16mol%。在较宽的温度范围内测定了这两种溶液中冰晶外表面的生长速率。发现生长速率通常与时间无关，尤其是在较低温度下。甘油溶液中冰晶的生长速率在比Me2SO溶液显著更高的温度下变得可以忽略不计。发现添加浓度为1.7和9.9mg g-1的冬季比目鱼抗冻蛋白对50w/w%甘油溶液中的冰晶生长速率没有显著影响。
