Fouret P J, Dabit D, Mergui J L, Uzan S
Faculté de Médecine Saint-Antoine, Université Paris VI, France.
Pathobiology. 1998;66(6):306-10. doi: 10.1159/000028038.
Losses of heterozygosity on the short arm of chromosome 3p are common in cervical carcinomas in the 3p13-3p21 region, and can be observed in intra-epithelial lesions accompanying cervical cancers. As a preliminary attempt to determine whether these losses can be observed in intra-epithelial cervical lesions without concomitant invasive carcinoma, we have used two microsatellite markers located at the two most frequently deleted segments of the 3p13-3p21 region. We have studied 36 cases of grade II and grade III cervical intra-epithelial neoplasias obtained by conisation biopsies and 30 cases of cervical carcinoma including 3 micro-invasive squamous cell carcinomas. We found loss of heterozygosity or microsatellite instability in 6 of 16 (38%) and 9 of 23 (39%) informative cases of cervical carcinoma at 3p13 and 3p21, respectively. Four of 27 (15%) cases of cervical intra-epithelial neoplasia showed loss of heterozygosity at 3p13, whereas loss of heterozygosity or microsatellite instability at 3p21 was found in 5 of 19 cases (26%). No relationship was found between 3p loss of heterozygosity and human papillomavirus infection. In conclusion, losses of heterozygosity at 3p13 and 3p21 occur in premalignant lesions without concomitant invasive lesions. The prevalence and precise extent of these losses should be established by a more extensive analysis.
3号染色体短臂杂合性缺失在3p13 - 3p21区域的宫颈癌中很常见，并且在宫颈癌伴发的上皮内病变中也可观察到。作为确定这些缺失是否能在无浸润性癌的宫颈上皮内病变中观察到的初步尝试，我们使用了位于3p13 - 3p21区域两个最常缺失片段的两个微卫星标记。我们研究了通过锥切活检获得的36例II级和III级宫颈上皮内瘤变病例以及30例宫颈癌病例，其中包括3例微浸润性鳞状细胞癌。我们分别在3p13和3p21的16例（38%）和23例（39%）信息丰富的宫颈癌病例中发现了杂合性缺失或微卫星不稳定性。27例宫颈上皮内瘤变病例中有4例（15%）在3p13处显示杂合性缺失，而19例病例中有5例（26%）在3p21处发现杂合性缺失或微卫星不稳定性。未发现3p杂合性缺失与人类乳头瘤病毒感染之间存在关联。总之，3p13和3p21处的杂合性缺失发生在无浸润性病变的癌前病变中。这些缺失的发生率和确切范围应通过更广泛的分析来确定。
