[用于鉴定肺炎链球菌不可分型菌株的推测性试验和分子杂交]

[Presumptive tests and molecular hybridization for the identification of untypable strains of Streptococcus pneumoniae].

作者信息

Schlegel L, Prostak J M, Spicq C, Sissia G, Frémaux A, Geslin P

机构信息

Centre National de Référence des Pneumocoques, Centre Hospitalier Intercommunal, Créteil, France.

出版信息

Pathol Biol (Paris). 1998 Jun;46(6):459-63.

PMID:9769882

Abstract

Five different methods for identification of pneumococci (optochine susceptibility, bile solubility, Slidex Pneumo-kit, Phadebact Pneumococcus test, AccuProbe DNA test) were evaluated with a total of 280 Streptococcus pneumoniae non typable strains. 189 strains were identified as pneumococci according to the AccuProbe test results. Among these, 180 strains (95.2%) were optochine sensitive (d > or = 12 mm). Bile solubility was seen in 125 (66.1%) of the pneumococci. Immunological identifications were respectively positive for 67 and 56 among 140 strains. By comparison with the DNA/RNA reassociation method, the poor sensitivities and specificities of the presumptive identification tests are actually demonstrated for pneumococcal non typable strains. Thus, the AccuProbe DNA test is seen as the only adequate method for identification of such strains.

摘要

使用总共280株不可分型的肺炎链球菌菌株，对五种不同的肺炎球菌鉴定方法（奥普托欣敏感性试验、胆汁溶解试验、Slidex肺炎球菌试剂盒、Phadebact肺炎球菌试验、AccuProbe DNA试验）进行了评估。根据AccuProbe试验结果，189株菌株被鉴定为肺炎球菌。其中，180株（95.2%）对奥普托欣敏感（抑菌圈直径d≥12mm）。125株（66.1%）肺炎球菌表现出胆汁溶解现象。在140株菌株中，免疫鉴定分别有67株和56株呈阳性。与DNA/RNA重缔合方法相比，对于不可分型的肺炎球菌菌株，推定鉴定试验的敏感性和特异性实际上较低。因此，AccuProbe DNA试验被视为鉴定此类菌株的唯一合适方法。