[新鲜、冷冻保存或液体培养基保存的人甲状旁腺腺瘤的抗原性]

[Antigenicity of fresh, cryopreserved, or liquid-medium-preserved human parathyroid adenomas].

作者信息

Martin L, Viennet G, Racadot E, Saint Hillier Y, Carbillet J P

机构信息

Laboratoire d'Anatomie et de Cytologie Pathologiques, Hôpital Jean Minjoz, Besançon, France.

出版信息

Pathol Biol (Paris). 1998 May;46(5):315-8.

PMID:9769891

Abstract

We have studied the ability of the cryopreservation and culture techniques to reduce the antigenicity of human parathyroid tissue by suppressing HLA DR bearing cells. Antigenicity was studied with an immunoperoxidase technique applied on frozen sections. Antibody against HLA DR, CD1a, CD3, CD22, CD45RA, CD68 and H et Y antigens were used. In fresh parathyroid tissue, endothelial cells, histiocytes and interstitial dendritic cells expressed HLA DR antigens. Antigenicity of cryopreserved tissue were not altered. In cultured tissue, interstitial HLA DR bearing cells have disappeared but antigenicity of endothelial cells were not modified.

摘要

我们研究了冷冻保存和培养技术通过抑制携带HLA - DR的细胞来降低人甲状旁腺组织抗原性的能力。采用免疫过氧化物酶技术对冰冻切片进行研究以检测抗原性。使用了针对HLA - DR、CD1a、CD3、CD22、CD45RA、CD68以及H和Y抗原的抗体。在新鲜甲状旁腺组织中，内皮细胞、组织细胞和间质树突状细胞表达HLA - DR抗原。冷冻保存组织的抗原性未改变。在培养的组织中，携带HLA - DR的间质细胞消失了，但内皮细胞的抗原性未改变。