McMurry L M, Oethinger M, Levy S B
Center for Adaptation Genetics and Drug Resistance, Tufts University School of Medicine, Boston, MA 02111, USA.
FEMS Microbiol Lett. 1998 Sep 15;166(2):305-9. doi: 10.1111/j.1574-6968.1998.tb13905.x.
Triclosan (Irgasan) is a broad spectrum antimicrobial agent used in handsoaps, toothpastes, fabrics, and plastics. It inhibits lipid biosynthesis in Escherichia coli, probably by action upon enoyl reductase (FabI) (McMurry, L.M., Oethinger, M. and Levy, S.B. (1988) Nature 394, 531-532). We report here that overexpression of the multidrug efflux pump locus acrAB, or of marA or soxS, both encoding positive regulators of acrAB, decreased susceptibility to triclosan 2-fold. Deletion of the acrAB locus increased the susceptibility to triclosan approximately 10-fold. Four of five clinical E. coli strains which overexpressed marA or soxS also showed enhanced triclosan resistance. The acrAB locus was involved in the effects of triclosan upon both cell growth rate and cell lysis.
三氯生（玉洁新）是一种广谱抗菌剂，用于洗手液、牙膏、织物和塑料制品中。它可能通过作用于烯酰还原酶（FabI）来抑制大肠杆菌中的脂质生物合成（麦克默里，L.M.，厄廷格，M.和利维，S.B.（1988年）《自然》394，531 - 532）。我们在此报告，多药外排泵基因座acrAB或marA或soxS（两者均编码acrAB的正调控因子）的过表达使对三氯生的敏感性降低了2倍。acrAB基因座的缺失使对三氯生的敏感性增加了约10倍。过表达marA或soxS的五株临床大肠杆菌菌株中有四株也表现出对三氯生的抗性增强。acrAB基因座参与了三氯生对细胞生长速率和细胞裂解的影响。
