Srinivasan R, Poulsom R, Hurst H C, Gullick W J
Receptor Biology Laboratory, Imperial Cancer Research Fund, Imperial College School of Medicine, Hammersmith Hospital, London, U.K.
J Pathol. 1998 Jul;185(3):236-45. doi: 10.1002/(SICI)1096-9896(199807)185:3<236::AID-PATH118>3.0.CO;2-7.
The c-erbB-4/HER4 receptor belongs to the family of the type I growth factor receptors. Mouse monoclonal antibodies have been raised to the cytoplasmic domain of the c-erbB-4 receptor and characterized; the antibody HFR-1 has been used to determine the pattern of expression of the c-erbB-4 protein immunohistochemically in formalin-fixed, paraffin-embedded adult and fetal tissues. The expression of c-erbB-4 mRNA was determined by using 35S-labelled riboprobes and tissue in situ hybridization. c-erbB-4 is widely expressed in many adult and fetal tissues, including the lining epithelia of the gastrointestinal, urinary, reproductive, and respiratory tracts, as well as the skin, skeletal muscle, circulatory, endocrine, and nervous systems. The developing brain and heart notably express high levels of this receptor. The pattern of c-erbB-4 protein expression is also reported in a survey of common solid human cancers. Loss of expression was noted in 40-80 per cent of adenocarcinomas and up to 100 per cent of squamous cell carcinomas, whereas overexpression was observed in about 10-20 per cent of adenocarcinomas and astrocytomas. In general, the pattern of c-erbB-4 expression in normal tissues and cancers suggests that it tends to be associated with the differentiated compartment.
c-erbB-4/HER4受体属于I型生长因子受体家族。已制备出针对c-erbB-4受体胞质结构域的小鼠单克隆抗体并进行了特性鉴定；抗体HFR-1已用于通过免疫组织化学方法确定c-erbB-4蛋白在福尔马林固定、石蜡包埋的成人及胎儿组织中的表达模式。通过使用35S标记的核糖探针和组织原位杂交来确定c-erbB-4 mRNA的表达。c-erbB-4在许多成人和胎儿组织中广泛表达，包括胃肠道、泌尿道、生殖道和呼吸道的内衬上皮，以及皮肤、骨骼肌、循环系统、内分泌系统和神经系统。发育中的脑和心脏尤其高表达这种受体。在一项对常见人类实体癌的调查中也报告了c-erbB-4蛋白的表达模式。在40%-80%的腺癌和高达100%的鳞状细胞癌中观察到表达缺失，而在约10%-20%的腺癌和星形细胞瘤中观察到过表达。一般来说，c-erbB-4在正常组织和癌症中的表达模式表明它往往与分化区室相关。
