Kall M A, Hellström I, Hellström K E
Int J Cancer. 1976 Oct 15;18(4):488-97. doi: 10.1002/ijc.2910180414.
Mixtures of lymph node and spleen cells from normal (untreated) BALB/c mice and from BALB/c mice whose syngeneic tumors had been excised 7-28 days previously ("tumor-excised mice"), were sensitized in vitro by cultivation for 9 days with cells from syngeneic, methylcholanthrene-induced sarcomas. The in vitro-sensitized lymphoid cells were tested in a 36-h microcytotoxicity assay for reactivity against target cells carrying the sensitizing tumor antigens, as well as against control target cells lacking these antigens. After co-cultivation with tumor cells, lymphoid cells from both normal and tumor-excised mice were cytotoxic to tumor cells carrying the sensitizing antigens. The cytotoxicity was generally specific, and occurred at low effector: target cell ratios (in some experiments down to 1:1). When lymphoid cells from tumor-excised mice were exposed in vitro for 9 days to cells carrying the same antigens as those which were originally present on the surgical excised tumors, the effector cells obtained gave a dose-dependent cytotoxic response suggestive of a linear relationship. When lymphoid cells from normal mice were similarly sensitized for 9 days, specifically cytotoxic lymphoid cells were generated but no linear dose-dependent response was detected.
将来自正常（未处理）BALB/c小鼠以及同基因肿瘤在7 - 28天前已切除的BALB/c小鼠（“肿瘤切除小鼠”）的淋巴结和脾细胞混合物，与来自同基因、甲基胆蒽诱导肉瘤的细胞一起体外培养9天进行致敏。在36小时的微细胞毒性试验中测试体外致敏的淋巴细胞对携带致敏肿瘤抗原的靶细胞以及对缺乏这些抗原的对照靶细胞的反应性。与肿瘤细胞共培养后，来自正常小鼠和肿瘤切除小鼠的淋巴细胞对携带致敏抗原的肿瘤细胞均具有细胞毒性。这种细胞毒性通常具有特异性，并且在低效应细胞：靶细胞比例下发生（在一些实验中低至1:1）。当将来自肿瘤切除小鼠的淋巴细胞在体外与携带与手术切除肿瘤上最初存在的抗原相同的抗原的细胞接触9天时，获得的效应细胞产生剂量依赖性细胞毒性反应，提示存在线性关系。当将来自正常小鼠的淋巴细胞以类似方式致敏9天时，产生了特异性细胞毒性淋巴细胞，但未检测到线性剂量依赖性反应。
