Zöller M, Price M R, Baldwin R W
Int J Cancer. 1975 Oct 15;16(4):593-606. doi: 10.1002/ijc.2910160409.
Cell-mediated immune reactions against carcinogen-induced rat tumours were demonstrated by the in vitro microcytotoxicity test. Lymph-node cells from syngeneic rats immunized against individual aminoazo-dye-induced hepatomas or 3-methylcholanthrene-induced sarcomas were cytotoxic for cells of the immunizing tumour compared with the effects of normal lymph-node cells. Only a low level of cross-reactivity was detectable against target cells derived from otherr tumours. Conversely, lymph-node cells from tumour -bearing rats were cross-reactive against a range of tumour cells, suggesting that the tumour-bearer was sensitized against a common antigen(s) as well as the individually specific antigen associated with these tumours. Lymph-node cells were reactive at an early time (from day 2) after tumour implantation and their cytotoxic potential persisted until terminal stages of tumour growth. Spleen cells from tumour-bearer and tumour-immune donors also exhibited comparable cytotoxic reactivities of the same specificity as that shown by lymph-node cell preparations. These findings are discussed in relation to the development of cell-mediated immune responses in the tumour-bearing rat and in immunized rats which are capable of rejecting transplanted tumour cells.
通过体外微细胞毒性试验证明了针对致癌物诱导的大鼠肿瘤的细胞介导免疫反应。与正常淋巴结细胞的作用相比，来自同种系大鼠的、针对单个氨基偶氮染料诱导的肝癌或3-甲基胆蒽诱导的肉瘤免疫的淋巴结细胞对免疫肿瘤的细胞具有细胞毒性。对源自其他肿瘤的靶细胞仅可检测到低水平的交叉反应性。相反，来自荷瘤大鼠的淋巴结细胞对一系列肿瘤细胞具有交叉反应性，这表明荷瘤者不仅对与这些肿瘤相关的个体特异性抗原敏感，而且对一种共同抗原敏感。淋巴结细胞在肿瘤植入后的早期（从第2天开始）就有反应，并且它们的细胞毒性潜力一直持续到肿瘤生长的末期。来自荷瘤者和肿瘤免疫供体的脾细胞也表现出与淋巴结细胞制剂相同特异性的可比细胞毒性反应性。结合在荷瘤大鼠和能够排斥移植肿瘤细胞的免疫大鼠中细胞介导免疫反应的发展对这些发现进行了讨论。
