Luo F
Department of Physiology, Beijing Medical University.
Sheng Li Ke Xue Jin Zhan. 1996 Jul;27(3):241-4.
Clinical and animal studies were performed to evaluate the effect of repeated transcutaneous electric nerve stimulation (TENS) applied on acupoints of pain patients and the effect of repeated electroacupuncture (EA) at acupoints of the rat on experimental arthritic pain models. Attempts were also made to evaluate whether substance P (SP), cholecystokinin octopeptide (CCK-8), and met5-enkephalin (MEK) are involved in the mechanism of the cumulative effect of repeated EA on experimental arthritis using neuropharmacological (receptor antagonists) and neurochemical (radioimmunoassay, RIA) approach. The main results show that repeated TENS of 100 Hz relieves spinal spasticity in a cumulative manner, while TENS of 2/15 Hz is effective in relieving chronic pain. In animal chronic pain models, repeated EA suppressed the hyperalgesia in arthritic rats in a cumulative manner. Further studies revealed that in pain patients there was a plastic change in the release and metabolic rate of spinal opioid peptides. In arthritic rats there was also a change in the releasing rate of spinal SP, CCK-8, and MEK as compared to control rats, an effect modulated by repeated EA. These plastic changes occured under chronic pain condition and their modulation by repeated acupoint stimulation may explain the mechanisms of the cumulative effect of acupuncture on chronic pain.
开展了临床和动物研究，以评估对疼痛患者穴位进行重复经皮电神经刺激（TENS）的效果，以及对大鼠穴位进行重复电针（EA）对实验性关节炎疼痛模型的效果。还尝试使用神经药理学（受体拮抗剂）和神经化学（放射免疫分析，RIA）方法，评估P物质（SP）、胆囊收缩素八肽（CCK-8）和甲硫氨酸脑啡肽（MEK）是否参与重复电针对实验性关节炎的累积效应机制。主要结果表明，100Hz的重复TENS以累积方式缓解脊髓痉挛，而2/15Hz的TENS有效缓解慢性疼痛。在动物慢性疼痛模型中，重复电针以累积方式抑制关节炎大鼠的痛觉过敏。进一步研究表明，疼痛患者脊髓阿片肽的释放和代谢率发生了可塑性变化。与对照大鼠相比，关节炎大鼠脊髓中SP、CCK-8和MEK的释放率也发生了变化，这种效应受重复电针调节。这些可塑性变化发生在慢性疼痛状态下，重复穴位刺激对其的调节可能解释了针灸对慢性疼痛累积效应的机制。
