Li J, Xia X, Hua D, Wang W, Chen Z, Ruan C
Jiangsu Institute of Hematology, Affiliated First Hospital, Suzhou Medical College, China.
Chin Med J (Engl). 1997 Dec;110(12):919-22.
To evaluate the characteristics of P-glycoprotein (P-gp) expression of acute non-lymphocytic leukemia (ANLL) at different status and the prognostic and biological features in ANLL at diagnosis.
Monoclonal antibody UIC2 and indirect immunofluorescence assay by flow cytometry were used to determine P-gp expression of 169 patients with ANLL, including 152 previously untreated, 7 refractory and 10 at remission.
P-gp was expressed in 28.9% of the previously untreated ANLL cases and P-gp was lower than that in 71.4% of the refractory cases (P < 0.05). No P-gp expression was found in the patients at remission. For previously untreated AN-LL, P-gp was highly expressed in hybrid acute leukemia (66.7%) and acute monoblastic leukemia (47.4%). P-gp expression was highly associated with surface markers [cluster of differentiation (CD) 34, CD7, CD14, CD42b and CD61] and unfavorable cytogenetic abnormalities. About 23% of P-gp-ANLL obtained complete remission, which was significantly lower than that (76%) in P-gp-cases.
P-gp expression is higher in refractory ANLL cases than that in cases at diagnosis or at remission. P-gp is an index of poor prognosis in adults with ANLL. P-gp+ ANLL cases have unique clinical and biological characteristics.
评估急性非淋巴细胞白血病（ANLL）在不同状态下P-糖蛋白（P-gp）的表达特征以及诊断时ANLL的预后和生物学特征。
采用单克隆抗体UIC2和流式细胞术间接免疫荧光法检测169例ANLL患者的P-gp表达，其中152例为初治患者，7例为难治性患者，10例为缓解期患者。
初治ANLL患者中28.9%表达P-gp，其表达低于71.4%的难治性患者（P<0.05）。缓解期患者未发现P-gp表达。对于初治AN-LL，P-gp在混合性急性白血病（66.7%）和急性单核细胞白血病（47.4%）中高表达。P-gp表达与表面标志物[分化簇（CD）34、CD7、CD14、CD42b和CD61]及不良细胞遗传学异常高度相关。约23%的P-gp阴性ANLL患者获得完全缓解，显著低于P-gp阳性患者（76%）。
难治性ANLL患者的P-gp表达高于诊断时或缓解期患者。P-gp是成人ANLL预后不良的指标。P-gp阳性ANLL患者具有独特的临床和生物学特征。
