Tu H, Wen Y, Xiong S
Department of Molecular Virology, Shanghai Medical University.
Zhonghua Yi Xue Za Zhi. 1997 Aug;77(8):571-4.
To study the mutations in the core promoter (CP) region of hepatitis B virus (HBV) in Chinese viral hepatits B patients.
CP regions of 48 HBV strains were analysed by polymerase chain reaction (PCR) direct sequencing approach.
67% of the samples were detected to have point mutations in the CP region. The hot spots located in nt 1754-1766 and nt 1801-1811 while nucleotides in nt 1777-1800 and nt 1812-1836 were highly conserved. nt. 1764 mutation was present in HBeAg negative patients. There were 16 point mutations in region overlapping X gene, 9 of which lead to amino acid change.
Though mutations in CP region of HBV appear frequently, the sequences associated with viral transcription are rarely changed. Point mutation at nt 1764 is related to HBeAg negative phenotype, but it is not the specific mutation of fulminant hepatitis. The importance of mutations in the X gene overlapping region needs to be further investigated.
研究中国乙肝患者中乙型肝炎病毒（HBV）核心启动子（CP）区域的突变情况。
采用聚合酶链反应（PCR）直接测序法分析48株HBV毒株的CP区域。
67%的样本在CP区域检测到点突变。热点位于核苷酸1754 - 1766和核苷酸1801 - 1811，而核苷酸1777 - 1800和核苷酸1812 - 1836高度保守。核苷酸1764突变存在于HBeAg阴性患者中。X基因重叠区域有16个点突变，其中9个导致氨基酸改变。
虽然HBV的CP区域突变频繁出现，但与病毒转录相关的序列很少改变。核苷酸1764的点突变与HBeAg阴性表型相关，但它不是暴发性肝炎的特异性突变。X基因重叠区域突变的重要性需要进一步研究。
