Lu W, Tang D, Cao S
Department of Surgery, Tongji Hospital, Tongji Medical University, Wuhan.
Zhonghua Yi Xue Za Zhi. 1997 Nov;77(11):812-4.
To study on the expression of calponin in an animal model of gallstone disease and investigate the molecular mechanisms of gallstone formation.
After feeding a high-cholesterol diet to guinea pigs, Oddi's sphincters were disseced on day 30 and day 60 respectively. We used RT-PCR, western-blotting to evaluate expression level of calponin gene.
Down-regulation of calponin gene expression was observed in animals with gallstone. The levels of both protein and mRNA expression for calponin on day 30 and day 60 were lower than those of control group with the level from day 60 lower than that from day 60, while myosin expressions were relatively stable.
Our results indicated that the decrease of calponin could increase the pressure of sphincter of Oddi, aggravate the stasis of bile and promote the gallstone formation.
研究钙调蛋白在胆结石疾病动物模型中的表达，探讨胆结石形成的分子机制。
给豚鼠喂食高胆固醇饮食，分别在第30天和第60天解剖Oddi括约肌。我们使用逆转录聚合酶链反应（RT-PCR）、蛋白质免疫印迹法来评估钙调蛋白基因的表达水平。
在患有胆结石的动物中观察到钙调蛋白基因表达下调。第30天和第60天钙调蛋白的蛋白质和信使核糖核酸（mRNA）表达水平均低于对照组，且第60天的水平低于第30天，而肌球蛋白表达相对稳定。
我们的结果表明，钙调蛋白的减少会增加Oddi括约肌压力，加重胆汁淤积并促进胆结石形成。
