Youming Ding, Bin Wang, Weixing Wang, Binghua Wang, Ruoyu Luo, Bangchang Cheng
Department of Hepatobiliary and Laparoscopic Surgery, Renmin Hospital of Wuhan University, Wuhan, China.
Saudi Med J. 2006 Nov;27(11):1661-6.
To explore the effect of h(1) calponin mRNA expression on the biliary tract dynamics, and investigate the molecular mechanisms of gallstone formation in pregnancy.
This study was carried out in Renmin Hospital of Wuhan University, and in the Department of Molecular Biology and Biochemistry, Wuhan University School of Medicine, Wuhan, China from July to December 2004. Thirty female guinea pigs were divided randomly into 3 groups, the nonpregnant group (n=10) (group A), the 30 days of pregnancy group (n=10) (group B), and the 60 days of pregnancy group (n=10) (group C). Animal models of pregnancy were established on pregnant group guinea pigs through feeding animals with one cage according to female versus male as 4:1. The total cholesterol (TC), total bilirubin (TBiL), total bile acid (TBA) in the bile and the serum estradiol (E(2)), progesterone (Pg) levels were determined respectively. Expression levels of h(1) calponin mRNA in gallbladder smooth muscles and Oddi's Sphincter (OS) were evaluated using semiquantitative reverse transcription polymerase chain reaction (RT-PCR).
The concentration of TC, TBiL and the serum E(2) and Pg were more significantly increasing in group C than that in the other 2 groups. However, the concentration of TBA decreased gradually from group A to group C. Up-regulation of h(1) calponin gene expression was observed in the gallbladder smooth muscles in group C, but converse in OS.
The h(1) calponin might play an important role in inducing dysfunction of extrahepatic biliary tract, bile stasis in gallbladder and gallstone formation in pregnancy.
探讨h(1) 钙调蛋白mRNA表达对胆道动力学的影响，并研究妊娠期胆结石形成的分子机制。
本研究于2004年7月至12月在武汉大学人民医院及武汉大学医学院分子生物学与生物化学系开展。30只雌性豚鼠随机分为3组，未孕组（n = 10）（A组）、妊娠30天组（n = 10）（B组）和妊娠60天组（n = 10）（C组）。妊娠组豚鼠通过按4:1的雌雄比例同笼饲养建立妊娠动物模型。分别测定胆汁中的总胆固醇（TC）、总胆红素（TBiL）、总胆汁酸（TBA）以及血清雌二醇（E(2)）、孕酮（Pg）水平。采用半定量逆转录聚合酶链反应（RT-PCR）评估胆囊平滑肌和Oddi括约肌（OS）中h(1) 钙调蛋白mRNA的表达水平。
C组的TC、TBiL浓度以及血清E(2)、Pg水平较其他两组显著升高。然而，TBA浓度从A组到C组逐渐降低。C组胆囊平滑肌中观察到h(1) 钙调蛋白基因表达上调，但在OS中则相反。
h(1) 钙调蛋白可能在妊娠期肝外胆道功能障碍、胆囊胆汁淤积及胆结石形成中起重要作用。
