Cai S T, Zhou F L, Zhang J Z
Institute of Biophysics, Academia Sinica, Beijing.
Shi Yan Sheng Wu Xue Bao. 1996 Dec;29(4):357-63.
In order to investigate relationship between vimentin and nuclear pore complex, we examined binding ability of vimentin, expressed in E. coli, with nucleoporin, isolated from rat liver nuclei, in vitro. Negative staining electron microscopy showed that the vimentin expressed in bacteria assembled 10 nm filament in vitro. SDS-PAGE and western blotting showed that Nup 180 bind to vimentin in binding assay in vitro. Combining immunogold labeling and negative staining electron microscopy techniques, we showed that Nup 180 bind on the 10 nm vimentin filaments. The experiment results indicated that vimentin filament may be anchored on nuclear pore complex in vivo by binding with Nup 180.
为了研究波形蛋白与核孔复合体之间的关系，我们在体外检测了在大肠杆菌中表达的波形蛋白与从大鼠肝细胞核中分离出的核孔蛋白的结合能力。负染色电子显微镜显示，细菌中表达的波形蛋白在体外组装成10纳米的细丝。SDS-PAGE和蛋白质免疫印迹表明，在体外结合试验中核孔蛋白180与波形蛋白结合。结合免疫金标记和负染色电子显微镜技术，我们发现核孔蛋白180结合在10纳米的波形蛋白细丝上。实验结果表明，波形蛋白细丝在体内可能通过与核孔蛋白180结合而锚定在核孔复合体上。
