Cai S T, Zhou F L, Zhang J Z
Institute of Biophysics Academia Sinica, Beijing.
Shi Yan Sheng Wu Xue Bao. 1997 Jun;30(2):193-9.
The relationship between intermediate filament and nucleus is an important question to be solved. By combining sequential cell fractionation with immunoblotting, we showed that the intermediate filament protein in turkey erythrocyte is vimentin. Using pre-embedment immunogold labeling techniques together with sequential cell fractionation, we showed that cytoplasmic intermediate filaments in turkey erythrocyte are labeled specifically by rabbit anti-vimentin antibody-protein A-gold. Moreover, we showed that the cytoplasmic filaments anchored on nuclear pore complex were also labeled by rabbit anti-vimentin antibody-protein A-gold specifically. Our results demonstrated that cytoplasmic filaments anchored on nuclear pore complex was vimentin filament. The experiments indicated that vimentin filament may be anchored on nuclear pore complex by binding with Nup 180 and intermediate filament may be involved in nuclear transportation.
中间丝与细胞核之间的关系是一个有待解决的重要问题。通过将连续细胞分级分离与免疫印迹相结合，我们发现火鸡红细胞中的中间丝蛋白是波形蛋白。利用包埋前免疫金标记技术与连续细胞分级分离，我们发现兔抗波形蛋白抗体-蛋白A-金可特异性标记火鸡红细胞中的细胞质中间丝。此外，我们还发现锚定在核孔复合体上的细胞质丝也被兔抗波形蛋白抗体-蛋白A-金特异性标记。我们的结果表明，锚定在核孔复合体上的细胞质丝是波形蛋白丝。这些实验表明，波形蛋白丝可能通过与核孔蛋白180结合而锚定在核孔复合体上，并且中间丝可能参与核运输。
