脂肪酸二乙醇酰胺中一乙醇胺和N-亚硝基二乙醇胺的测定

Determination of diethanolamine and N-nitrosodiethanolamine in fatty acid diethanolamides.

作者信息

Chou H J

机构信息

U.S. Food and Drug Administration, SW, Washington, DC 20204, USA.

出版信息

J AOAC Int. 1998 Sep-Oct;81(5):943-7.

PMID:9772735

Abstract

Diethanolamine (DEA) is a precursor of N-nitrosodiethanolamine (NDELA), an animal carcinogen. A gas chromatographic (GC) method was developed for determining DEA in fatty acid diethanolamides that are commonly used in cosmetic products. Methanolic solutions of the amides were analyzed by GC with flame ionization detection on either a wide-bore methyl silicone (Rtx-1) or 95% dimethyl--5% diphenyl polysiloxane (SPB-5) capillary column. Recovery of DEA from fatty acid dialkanolamides at fortification levels of 0.50, 1.00, and 5.00% ranged from 94 to 100%. In a survey of commercial fatty acid diethanolamides, DEA was found at levels ranging from 1.1 to 14.0%, and most were in good agreement with manufacturer's DEA specifications. Fatty acid diethanolamides also were anlayzed for NDELA by liquid chromatography interfaced to a thermal energy analyzer. Recovery of NDELA from fatty acid diethanolamides at fortification levels of 50, 100, and 200 ppb averaged 95%. No NDELA was found in any of the fatty acid diethanolamide samples analyzed.

摘要

二乙醇胺（DEA）是一种动物致癌物N-亚硝基二乙醇胺（NDELA）的前体。开发了一种气相色谱（GC）方法，用于测定化妆品中常用的脂肪酸二乙醇酰胺中的DEA。酰胺的甲醇溶液通过GC在宽口径甲基硅氧烷（Rtx-1）或95%二甲基-5%二苯基聚硅氧烷（SPB-5）毛细管柱上进行火焰离子化检测分析。在0.50%、1.00%和5.00%的强化水平下，从脂肪酸二烷醇酰胺中回收DEA的回收率为94%至100%。在对市售脂肪酸二乙醇酰胺的调查中，发现DEA的含量范围为1.1%至14.0%，大多数与制造商的DEA规格相符。脂肪酸二乙醇酰胺还通过与热能分析仪联用的液相色谱法分析NDELA。在50、100和200 ppb的强化水平下，从脂肪酸二乙醇酰胺中回收NDELA的平均回收率为95%。在所分析的任何脂肪酸二乙醇酰胺样品中均未发现NDELA。