Larminie C G, White R J
Division of Biochemistry and Molecular Biology, University of Glasgow, UK.
DNA Seq. 1998 Mar;9(1):49-58. doi: 10.3109/10425179809050025.
We have identified a putative gene within the Caenorhabditis elegans genome which has the potential to encode a protein homologous to BRF, an RNA polymerase III general transcription factor. The predicted protein shares very similar overall structure with human and yeast BRF. In particular, its N-terminal half comprises a zinc-ribbon motif and a TR domain which is also present in the cyclin box. The C. elegans protein is more similar to human BRF than to the yeast BRF proteins, as would be expected from an evolutionary standpoint. Alignment of the C. elegans protein with the four known BRF proteins reveals two blocks conserved between all five sequences within the diverged C-terminal region. Profile searches using these regions suggest that they may contain evolutionarily conserved motifs. These comparisons provide insight into the structure and function of an important transcription factor.
我们在秀丽隐杆线虫基因组中鉴定出一个假定基因，它有可能编码一种与BRF（一种RNA聚合酶III通用转录因子）同源的蛋白质。预测的蛋白质与人类和酵母BRF具有非常相似的整体结构。特别是，它的N端一半包含一个锌带基序和一个也存在于细胞周期蛋白盒中的TR结构域。从进化的角度来看，秀丽隐杆线虫的蛋白质与人类BRF的相似性高于与酵母BRF蛋白质的相似性。秀丽隐杆线虫蛋白质与四种已知BRF蛋白质的比对揭示了在分化的C端区域内所有五个序列之间保守的两个区域。使用这些区域进行的轮廓搜索表明它们可能包含进化上保守的基序。这些比较为一种重要转录因子的结构和功能提供了深入了解。
