Shah A K, Stewart P L
Department of Molecular & Medical Pharmacology and Crump Institute for Biological Imaging, UCLA School of Medicine, Los Angeles, California, 90095-1770, USA.
J Struct Biol. 1998 Sep;123(1):17-21. doi: 10.1006/jsbi.1998.4011.
The QVIEW image-processing software package provides a graphical user interface for interactive and rapid selection of particles from digital electron micrographs. This package was developed to facilitate the selection of elliptical, hydrophobic, and aggregating particles, which present complications for other particle selection packages. Speed improvements have been attained by using the fast graphics routines available in the Tc1/Tk toolkit and by separating the time consuming image-processing procedures from the interactive selection steps. The software is based on C++ and Tc1/Tk, both of which are readily available on many computer platforms. Processing features include removal of oversaturated pixels, planar subtraction using three or more background regions, exclusion of unwanted density such as nearby particles, and application of a circular or elliptical mask. Particles, along with their corresponding background and exclude regions, may be selected interactively from multiple digital micrographs, and the selection parameters are saved in a disk file for later processing. C shell processing scripts are easily modified and customized for specific applications. In summary, this software package enables the user-friendly, rapid selection of particles suitable for input into three-dimensional reconstruction packages.
QVIEW图像处理软件包提供了一个图形用户界面，用于从数字电子显微照片中交互式快速选择颗粒。开发这个软件包是为了便于选择椭圆形、疏水性和聚集性颗粒，而这些颗粒对于其他颗粒选择软件包来说存在复杂性。通过使用Tc1/Tk工具包中可用的快速图形例程，以及将耗时的图像处理程序与交互式选择步骤分开，实现了速度提升。该软件基于C++和Tc1/Tk，这两者在许多计算机平台上都很容易获得。处理功能包括去除过饱和像素、使用三个或更多背景区域进行平面减法、排除不需要的密度（如附近的颗粒）以及应用圆形或椭圆形掩码。颗粒及其相应的背景和排除区域可以从多个数字显微照片中交互式选择，并且选择参数会保存在磁盘文件中以供后续处理。C shell处理脚本很容易针对特定应用进行修改和定制。总之，这个软件包能够实现用户友好、快速地选择适合输入到三维重建软件包中的颗粒。
