Lo Presti F, Riffard S, Vandenesch F, Etienne J
Centre National de Référence des Legionella, EA1655, Faculté de Médecine R. T. H. Laennec, 69372 Lyon Cedex 08, France.
J Clin Microbiol. 1998 Nov;36(11):3193-7. doi: 10.1128/JCM.36.11.3193-3197.1998.
Random amplified polymorphic DNA (RAPD) was used for the identification of Legionella species. Primer SK2 (5'-CGGCGGCGGCGG-3') and standardized RAPD conditions gave the technique a reproducibility of 93 to 100%, depending on the species tested. Species-specific patterns corresponding to the 42 Legionella species were consequently defined by this method; the patterns were dependent on the recognition of a core of common bands for each species. This specificity was demonstrated by testing 65 type strains and 265 environmental and clinical isolates. No serogroup-specific profiles were obtained. A number of unidentified Legionella isolates potentially corresponding to new species were clustered in four groups. RAPD analysis appears to be a rapid and reproducible technique for identification of Legionella isolates to the species level without further restriction or hybridization.
随机扩增多态性DNA(RAPD)被用于军团菌属的鉴定。引物SK2(5'-CGGCGGCGGCGG-3')和标准化的RAPD条件使该技术的重现性在93%至100%之间,这取决于所测试的菌种。通过该方法确定了与42种军团菌相对应的种特异性图谱;这些图谱依赖于对每种菌种共同条带核心的识别。通过对65株模式菌株以及265株环境和临床分离株进行测试,证实了这种特异性。未获得血清群特异性图谱。一些可能对应新菌种的未鉴定军团菌分离株被归为四组。RAPD分析似乎是一种快速且可重复的技术,可在无需进一步限制或杂交的情况下将军团菌分离株鉴定到种水平。