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嗜肺军团菌血清1型分型的任意引物聚合酶链反应、核糖体分型和单克隆抗体分析比较

Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.

作者信息

Gomez-Lus P, Fields B S, Benson R F, Martin W T, O'Connor S P, Black C M

机构信息

Departamento de Microbiologia y Medicina Preventiva y Salud Publica, Universidad de Zaragoza, Spain.

出版信息

J Clin Microbiol. 1993 Jul;31(7):1940-2. doi: 10.1128/jcm.31.7.1940-1942.1993.

DOI:10.1128/jcm.31.7.1940-1942.1993
PMID:8394380
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC265666/
Abstract

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis and ribotyping. Four strains representing the monoclonal antibody pattern most frequently associated with outbreaks all yielded unique amplicon patterns by AP-PCR.

摘要

采用任意引物聚合酶链反应（AP-PCR）对嗜肺军团菌血清1型进行特征分析。单个菌落的细胞可在数小时内通过AP-PCR进行亚型分型。通过AP-PCR对嗜肺军团菌血清1型菌株的鉴别与单克隆抗体分析和核糖分型相当。代表与暴发最常相关的单克隆抗体模式的4株菌株通过AP-PCR均产生了独特的扩增子模式。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/f493d48181ed/jcm00019-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/86ee32ad7596/jcm00019-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/1e975208fc2e/jcm00019-0279-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/f493d48181ed/jcm00019-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/86ee32ad7596/jcm00019-0279-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/1e975208fc2e/jcm00019-0279-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/71c7/265666/f493d48181ed/jcm00019-0280-a.jpg

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