Benson R F, Thacker W L, Daneshvar M I, Brenner D J
Childhood and Respiratory Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Int J Syst Bacteriol. 1996 Jul;46(3):631-4. doi: 10.1099/00207713-46-3-631.
Two Legionella-like organisms were isolated from water samples obtained in Adelaide, Australia. One organisms was isolated from a drinking water distribution system, and the other was isolated from a cooling tower at a sewage treatment plant. Both strains required L-cysteine for growth and contained cellular branched-chain fatty acids and ubiquinones typical of the genus Legionella. These strains were serologically distinct from each other as determined by a slide agglutination test. STrain 2074-AUS-ET (T = type strain) was serologically distinct from all previously described Legionella species and serotypes. Strain 2055-AUS-E could not be differentiated biochemically or serologically from Legionella quinlivanii. Both strains were shown by DNA hybridization studies (Hydroxyapatite method) to be members of new Legionella species. Legionella waltersii sp. nov. is the name proposed for strain 2074-AUS-ET (= ATCC 51914T). L. waltersii was less than 10% related to other Legionella species. Strain 2055-AUS-E (= ATCC 51913) was informally named Legionella genomospecies 1, since it could not be phenotypically distinguished from L. quinlivanii. Legionella genomospecies 1 was closely related to L. quinlivanii strains (53 to 69% related with 4.5 to 6.5% divergence at 60 degrees C and 31 to 52% related at 75 degrees C).
从澳大利亚阿德莱德采集的水样中分离出两种类军团菌生物。一种生物从饮用水分配系统中分离得到，另一种从污水处理厂的冷却塔中分离得到。两种菌株生长均需要L-半胱氨酸，并且含有军团菌属典型的细胞支链脂肪酸和泛醌。通过玻片凝集试验确定，这两种菌株在血清学上彼此不同。菌株2074-AUS-ET（T=模式菌株）在血清学上与所有先前描述的军团菌物种和血清型不同。菌株2055-AUS-E在生化或血清学上无法与昆氏军团菌区分开来。DNA杂交研究（羟基磷灰石法）表明，这两种菌株都是新军团菌物种的成员。沃尔特斯军团菌新种是为菌株2074-AUS-ET（=ATCC 51914T）提议的名称。沃尔特斯军团菌与其他军团菌物种的亲缘关系小于10%。菌株2055-AUS-E（=ATCC 51913）被非正式命名为军团菌基因种1，因为它在表型上无法与昆氏军团菌区分开来。军团菌基因种1与昆氏军团菌菌株密切相关（在60℃时相关性为53%至69%，差异为4.5%至6.5%；在75℃时相关性为31%至52%）。
