Calvo A, Matte C, Moullec J
Rev Fr Transfus Immunohematol. 1978 Mar;21(2):323-39. doi: 10.1016/s0338-4535(78)80067-1.
Groupamatic 360 can be adapted to the quantitative measurement of erythrocyte agglutination reactions. Here the authors give the necessary conditions to obtain the best sensitivity and the best reproductibility. They have considered globular suspensions, incubation of the erythrocyte antiserums mixtures, centrifugation, agitation, photometric measurement. As concerns the quantitative technique, we used the average peripheral measurement obtained in the twleve cuvettes of the same selected disc sector. The calibration is fixed to 50 mV for a non agglutinated red cell suspension and to 950 mV for albumin in a saline solution. In these conditions, the best discrimination is obtained for agglutination with measures between 400 and 800 mV. The maximum sensitivity is reached with reactions corresponding to 600 mV, that is to say in our selected conditions with 50% of free red cells. The choice of the zone between 500 and 600 mV enables to detect variations from 1.5 to 2% in an antibody solution concentration. This zone is particularly favorable for quantitative work. Although a part of these operations in this preliminary work were manually effected, the automatic centrifugation, agitation, photometric measurement and results print out make possible a large number of measurements with very satisfactory reproductibility conditions. Besides, these experiments pointed out some factors of improvement which will be helpful for the new 360 G Groupmatic equipments.
Groupamatic 360可用于红细胞凝集反应的定量测量。在此，作者给出了获得最佳灵敏度和最佳重现性的必要条件。他们考虑了球状悬浮液、红细胞抗血清混合物的孵育、离心、搅拌、光度测量。关于定量技术，我们使用了在同一选定圆盘扇区的十二个比色皿中获得的外周平均值测量。对于未凝集的红细胞悬浮液，校准值固定为50 mV，对于盐溶液中的白蛋白，校准值固定为950 mV。在这些条件下，对于400至800 mV之间的凝集测量，可获得最佳区分度。对应于600 mV的反应可达到最大灵敏度，也就是说在我们选定的条件下，有50%的游离红细胞。选择500至600 mV之间的区域能够检测抗体溶液浓度1.5%至2%的变化。该区域特别有利于定量工作。尽管在这项初步工作中的部分操作是手动进行的，但自动离心、搅拌、光度测量和结果打印使得在非常令人满意的重现性条件下进行大量测量成为可能。此外，这些实验指出了一些改进因素，这将有助于新型360 G Groupmatic设备。
