Beebee T J, Rowe G, Burke T
School of Biology, University of Sussex, Falmer, Brighton, UK.
Biochem Genet. 1998 Jun;36(5-6):219-28. doi: 10.1023/a:1018776925261.
A complete phylogeographic analysis of any species requires sampling throughout its biogeographical range. In the case of the natterjack toad Bufo calamita in Britain, recent local extinctions have left substantial areas of its historical range without extant populations. We therefore obtained tissue samples of archived Bufo calamita from four museums in the United Kingdom. A range of tissues (tongue, liver, skin, lung, and larval tail) was sampled from a total of 33 individual animals. DNA was extracted and eight polymorphic microsatellite loci were scored. One or more loci were amplified successfully from 27 individuals, and sufficient data were obtained from regions with few or no surviving populations to supplement a phylogeographic analysis based on extant populations.
对任何物种进行完整的系统地理学分析都需要在其生物地理范围内进行采样。就英国的黄条蟾蜍Bufo calamita而言，近期的局部灭绝使得其历史分布范围内的大片区域没有现存种群。因此，我们从英国的四家博物馆获取了存档的黄条蟾蜍的组织样本。从总共33只个体动物身上采集了一系列组织（舌头、肝脏、皮肤、肺和幼虫尾巴）。提取了DNA并对8个多态微卫星位点进行了评分。从27只个体中成功扩增出一个或多个位点，并从现存种群较少或没有现存种群的区域获得了足够的数据，以补充基于现存种群的系统地理学分析。
