Lounibos L P, Wilkerson R C, Conn J E, Hribar L J, Fritz G N, Danoff-Burg J A
Florida Medical Entomology Laboratory, University of Florida, Vero Beach 32962, USA.
J Med Entomol. 1998 Sep;35(5):830-8. doi: 10.1093/jmedent/35.5.830.
Based on similarity of male genitalia, the malaria vector Anopheles trinkae Faran from the eastern Andean piedmont of Colombia, Ecuador, Peru, and Bolivia was determined by Peyton (1993) to be a junior synonym of An. dunhami Causey, then known from a single locality in Amazonian Brazil. Following an appraisal of molecular, chromosomal, and morphological characters, we conclude herein that the 2 taxa are specifically distinct and remove An. trinkae from synonymy with An. dunhami. Eggs of the 2 species are distinguished easily by the anterior crown, long floats, and closed deck that occur only in An. trinkae. The X chromosome of larval polytenes is divisible into R and L arms in An. dunhami, but not in An. trinkae. A phenogram based on banding pattern scores from 18 random amplified polymorphic DNA primers separated with 100% resolution An. dunhami, An. trinkae, Anopheles nuneztovari Gabaldón and Anopheles darlingi Root. In the ITS2 region of rDNA, 25% of base sites distinguished An. trinkae from An. dunhami and 21% from the related An. nuneztovari; males of these 3 species had accessory glands of significantly different sizes. Preliminary isoenzyme screening indicated that 3 of 11 loci were diagnostic for separating An. trinkae from An. dunhami. The results indicate that An. dunhami is related more closely to An. nuneztovari than to An. trinkae and illustrate the merits of a multidisciplinary approach to mosquito systematics.
基于雄性生殖器的相似性，佩顿（1993年）确定来自哥伦比亚、厄瓜多尔、秘鲁和玻利维亚安第斯山东麓的疟疾媒介特林卡按蚊（Anopheles trinkae Faran）是邓氏按蚊（An. dunhami Causey）的次异名，当时邓氏按蚊仅在巴西亚马逊地区的一个地点被发现。在对分子、染色体和形态特征进行评估后，我们在此得出结论，这两个分类单元在物种上是不同的，并将特林卡按蚊从邓氏按蚊的异名中移除。这两个物种的卵很容易通过仅在特林卡按蚊中出现的前冠、长浮器和封闭甲板来区分。幼虫多线染色体的X染色体在邓氏按蚊中可分为R臂和L臂，而在特林卡按蚊中则不能。基于18个随机扩增多态性DNA引物的条带模式得分构建的系统发育树以100%的分辨率区分了邓氏按蚊、特林卡按蚊、努涅斯按蚊（Anopheles nuneztovari Gabaldón）和达林按蚊（Anopheles darlingi Root）。在核糖体DNA的ITS2区域，25%的碱基位点将特林卡按蚊与邓氏按蚊区分开来，21%的碱基位点将其与相关的努涅斯按蚊区分开来；这三个物种的雄性具有大小显著不同的副腺。初步的同工酶筛选表明，11个位点中的3个位点可用于区分特林卡按蚊和邓氏按蚊。结果表明，邓氏按蚊与努涅斯按蚊的关系比与特林卡按蚊的关系更密切，并说明了多学科方法在蚊虫系统学中的优点。
