Rosso G C, Masushige S, Warren C D, Kiorpes T C, Wolf G
J Biol Chem. 1976 Oct 25;251(20):6465-7.
Alkaline hydrolysis at 65 degrees converted D-mannosyl retinyl phosphate primarily into D-mannose 2-phosphate which was identified by its behavior on paper chromatography in two solvent systems, as well as by its resistance to acid hydrolysis under conditions that converted authentic alpha-D-mannosyl phosphate to mannose. When the D-mannosyl retinyl phosphate was hydrolyzed in alkali at 100 degrees, the main product was beta-D-mannosyl phosphate and not the alpha anomer (as shown by chromatography with authentic samples on thin layers of silica gel). No evidence for enzymatic hydrolysis of D-mannosyl retinyl phosphate by alpha-mannosidase was obtainable. It is concluded that the compound under investigation is beta-D-mannosyl retinyl phosphate.
在65摄氏度下进行碱性水解时，D-甘露糖基视黄基磷酸酯主要转化为2-磷酸-D-甘露糖，通过其在两种溶剂系统中的纸色谱行为以及在将纯α-D-甘露糖基磷酸酯转化为甘露糖的条件下对酸水解的抗性来鉴定。当D-甘露糖基视黄基磷酸酯在100摄氏度的碱中水解时，主要产物是β-D-甘露糖基磷酸酯而非α异头物（通过与纯样品在硅胶薄层上进行色谱分析显示）。未获得α-甘露糖苷酶对D-甘露糖基视黄基磷酸酯进行酶促水解的证据。得出的结论是，所研究的化合物是β-D-甘露糖基视黄基磷酸酯。
