由二磷酸鸟苷[14C]甘露糖和视黄基磷酸形成甘露糖基视黄基磷酸的酶的亚细胞定位。
Subcellular localization of the enzyme that forms mannosyl retinyl phosphate from guanosine diphosphate [14C]mannose and retinyl phosphate.
作者信息
Smith M J, Schreiber J B, Wolf G
出版信息
Biochem J. 1979 Jun 15;180(3):449-53. doi: 10.1042/bj1800449a.
Abstract
The subcellular distribution of the enzyme catalysing the conversion of retinyl phosphate and GDP-[14C]mannose into [14C]mannosyl retinyl phosphate was determined by using subcellular fractions of rat liver. Purity of fractions, as determined by marker enzymes, was 80% or better. The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02.
摘要
通过使用大鼠肝脏的亚细胞组分，确定了催化视黄基磷酸酯和GDP-[14C]甘露糖转化为[14C]甘露糖基视黄基磷酸酯的酶的亚细胞分布。通过标记酶测定，各组分的纯度为80%或更高。每个组分形成的甘露糖基视黄基磷酸酯的量（每毫克蛋白质的皮摩尔数/分钟）为：粗面内质网0.48±0.09（平均值±标准差）；光滑膜（由60%的光滑内质网和40%的高尔基体组成），0.18±0.03；高尔基体，0.13±0.03；质膜，0.02。
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引用本文的文献
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