Ishiguro N, Shinagawa M, Onoe S, Yamanouchi K, Saito T
Department of Veterinary Public Health, Obihiro University of Agriculture and Veterinary Medicine, Hokkaido, Japan.
Microbiol Immunol. 1998;42(8):579-82. doi: 10.1111/j.1348-0421.1998.tb02327.x.
A rapid method to determine the allelic variants of the sheep PrP gene was developed. DNA samples from 128 Suffolk sheep (39 rams and 89 ewes) were screened by using polymerase chain reactions and dot-blot hybridization with 32P-labeled nine allele-specific oligonucleotide probes corresponding to the polymorphic PrP codons 112, 136, 154 and 171. Three allelic variants of the PrP gene, PrP(MARQ), PrP(TARQ) and PrP(MARR), were found in the flocks. Among those variants, nearly half of the ewes had alleles of the 171-Arg variant that is closely associated with resistance to natural scrapie. Assessments of allelic mutations of the PrP gene may help to select the scrapie-resistant progenitors in the flocks.
开发了一种快速确定绵羊PrP基因等位变异体的方法。使用聚合酶链反应和与对应于多态性PrP密码子112、136、154和171的32P标记的9个等位基因特异性寡核苷酸探针进行点杂交，对128只萨福克羊（39只公羊和89只母羊）的DNA样本进行筛选。在这些羊群中发现了PrP基因的三种等位变异体，即PrP(MARQ)、PrP(TARQ)和PrP(MARR)。在这些变异体中，近一半的母羊具有与天然瘙痒病抗性密切相关的171-Arg变异体的等位基因。对PrP基因的等位基因突变进行评估可能有助于在羊群中选择抗瘙痒病的祖先。
