O'Rourke K I, Holyoak G R, Clark W W, Mickelson J R, Wang S, Melco R P, Besser T E, Foote W C
United States Department of Agriculture, Agricultural Research Service, Washington State University, Pullman 99164-7030, USA.
J Gen Virol. 1997 Apr;78 ( Pt 4):975-8. doi: 10.1099/0022-1317-78-4-975.
One-hundred and three Unites States Suffolk sheep were inoculated orally with a scrapie agent preparation and monitored for clinical disease and histopathological lesions characteristic of scrapie. A retrospective study of the polymorphisms at codon 171 of the prion protein (PrP) gene was performed on these sheep. All 63 sheep that developed scrapie during the observation period were homozygous for the glutamine 171 (171-QQ) PrP allele. Twelve 171-QQ sheep failed to develop disease. All 5 sheep homozygous for arginine (171-RR) and all 23 heterozygous (171-QR) sheep remained free of scrapie.
103只美国萨福克羊经口接种了一种瘙痒病病原体制剂，并对其进行监测，观察是否出现瘙痒病的临床症状和组织病理学损伤。对这些绵羊的朊病毒蛋白（PrP）基因第171密码子的多态性进行了回顾性研究。在观察期内出现瘙痒病的所有63只绵羊的谷氨酰胺171（171-QQ）PrP等位基因均为纯合子。12只171-QQ绵羊未发病。所有5只精氨酸纯合子（171-RR）绵羊和所有23只杂合子（171-QR）绵羊均未感染瘙痒病。
