Marcinkiewicz J, Grabowska A, Bereta J, Bryniarski K, Nowak B
Department of Immunology, Jagiellonian University Medical College, Cracow, Poland.
Immunopharmacology. 1998 Jul;40(1):27-38. doi: 10.1016/s0162-3109(98)00023-x.
We previously reported that taurine chloramine (TauCl), a product of activated neutrophils, inhibits the generation of macrophage inflammatory mediators such as nitric oxide (NO), TNF-alpha, and PGE2. Taurine, the most abundant free amino acid in the cytosol of neutrophils, is chlorinated to form TauCl by the halide-dependent myeloperoxidase (MPO) system. Under physiological conditions, TauCl reduces HOCl toxicity. In this study, we investigated the influence of TauCl on generation of oxygen free radicals, cytokines and eicosanoids by activated murine peritoneal neutrophils. We found that TauCl, but not taurine alone, inhibited the production of NO, prostaglandin E2, interleukin-6 and tumor necrosis factor-alpha, in a dose-dependent manner. In contrast, the products of the respiratory burst, as measured by luminol-dependent chemiluminescence (LCL), were reduced by both taurine and TauCl. However, taurine affected LCL at higher concentrations and to a lesser extent than TauCl. The results of these studies suggest that TauCl decreases production of tissue-damaging inflammatory mediators and may regulate the balance between protective, microbicidal and toxic effect of neutrophils.
我们先前报道过，活化中性粒细胞的产物牛磺酸氯胺（TauCl）可抑制巨噬细胞炎性介质的生成，如一氧化氮（NO）、肿瘤坏死因子-α（TNF-α）和前列腺素E2（PGE2）。牛磺酸是中性粒细胞胞质中含量最丰富的游离氨基酸，通过依赖卤化物的髓过氧化物酶（MPO）系统被氯化形成TauCl。在生理条件下，TauCl可降低次氯酸（HOCl）的毒性。在本研究中，我们调查了TauCl对活化的小鼠腹腔中性粒细胞产生氧自由基、细胞因子和类花生酸的影响。我们发现，TauCl而非单独的牛磺酸，以剂量依赖的方式抑制NO、前列腺素E2、白细胞介素-6和肿瘤坏死因子-α的产生。相比之下，通过鲁米诺依赖的化学发光（LCL）测定的呼吸爆发产物，牛磺酸和TauCl均可使其减少。然而，牛磺酸在较高浓度时影响LCL，且程度小于TauCl。这些研究结果表明，TauCl可减少组织损伤性炎性介质的产生，并可能调节中性粒细胞的保护、杀菌和毒性作用之间的平衡。
