Kim Hongyoon, Yoo Sunju, Lee Jung-Dae, Kim Hyang-Yeon, Kim Suhkmann, Kim Kyu-Bong
College of Pharmacy, Dankook University, 119 Dandae-ro, Cheonan 31116, Korea.
Center for Human Risk Assessment, Dankook University, 119 Dandae-ro, Cheonan 31116, Korea.
Metabolites. 2022 Jun 3;12(6):518. doi: 10.3390/metabo12060518.
Sulforaphane is an isocyanate abundantly present in cruciferous vegetables. In the present study, we aimed to investigate the effects of sulforaphane on secondhand smoking (SHS)-induced pulmonary damage in mice. Additionally, a metabolomic study was performed to identify biomarkers associated with pulmonary disease using proton nuclear magnetic resonance (H-NMR) analysis. Male C57BL6J mice were divided into a control group, an SHS exposure group (positive control group, PC), and a sulforaphane treatment group exposed to secondhand smoke (SS) ( = 5 per group). The PC and SS groups were exposed to secondhand smoke in a chamber twice daily for four weeks. Mice in the SS group were orally administered sulforaphane (50 mg/kg) for four weeks during secondhand smoke exposure. Histopathological examination of the lungs revealed pulmonary damage in PC mice, including loss of bronchial epithelial cells, bronchial wall thickening, and infiltration of macrophages. In contrast, mice in the SS group showed little or no epithelial thickening, thereby exhibiting reduced lung damage. Mouse serum and lung tissues were collected and analyzed to determine changes in endogenous metabolites using H-NMR. After target profiling, we identified metabolites showing the same tendency in the serum and lung as biomarkers for SHS-induced pulmonary damage, including taurine, glycerol, creatine, arginine, and leucine. As a result of histopathological examination, sulforaphane might inhibit SHS-induced lung damage, and metabolite analysis results suggest potential biomarkers for SHS-induced pulmonary damage in mice.
萝卜硫素是一种大量存在于十字花科蔬菜中的异氰酸酯。在本研究中，我们旨在探究萝卜硫素对小鼠二手烟（SHS）诱导的肺损伤的影响。此外，还进行了一项代谢组学研究，使用质子核磁共振（H-NMR）分析来鉴定与肺部疾病相关的生物标志物。将雄性C57BL6J小鼠分为对照组、SHS暴露组（阳性对照组，PC）和二手烟暴露的萝卜硫素治疗组（SS）（每组n = 5）。PC组和SS组每天在舱室内暴露于二手烟两次，持续四周。SS组的小鼠在二手烟暴露期间口服萝卜硫素（50 mg/kg），持续四周。肺组织病理学检查显示，PC组小鼠存在肺损伤，包括支气管上皮细胞丢失、支气管壁增厚和巨噬细胞浸润。相比之下，SS组小鼠几乎没有或没有上皮增厚，从而表现出肺损伤减轻。收集小鼠血清和肺组织，使用H-NMR分析以确定内源性代谢物的变化。经过目标分析，我们鉴定出在血清和肺中显示相同趋势的代谢物作为SHS诱导的肺损伤的生物标志物，包括牛磺酸、甘油、肌酸、精氨酸和亮氨酸。组织病理学检查结果表明，萝卜硫素可能抑制SHS诱导的肺损伤，代谢物分析结果提示了小鼠SHS诱导的肺损伤的潜在生物标志物。
