产色芽生菌病病原体——瓶霉属物种的核糖体DNA-限制性片段长度多态性分析及内转录间隔区1测序

rDNA-RFLP and ITS1 sequencing of species of the genus Fonsecaea, agents of chromoblastomycosis.

作者信息

Attili D S, De Hoog G S, Pizzirani-Kleiner A A

机构信息

Centraalbureau voor Schimmelcultures, Baarn, The Netherlands.

出版信息

Med Mycol. 1998 Aug;36(4):219-25.

PMID:9776838

Abstract

Amplicons of SSU and ITS1+2 rDNA of 13 strains of Fonsecaea pedrosoi and three strains of F. compacta were digested with seven restriction enzymes. In addition, the ITS1 region of 14 strains was sequenced. With both methods significant variation was found which, however, did not coincide with established species limits based on morphology.

摘要

对13株裴氏瓶霉和3株紧密瓶霉的小亚基（SSU）以及内转录间隔区1和2（ITS1+2）的核糖体DNA扩增子用7种限制性内切酶进行消化。此外，对14株菌株的ITS1区域进行了测序。两种方法均发现了显著差异，然而，这些差异与基于形态学确定的物种界限并不一致。