Abliz Paride, Fukushima Kazutaka, Takizawa Kayoko, Nieda Norikazu, Miyaji Makoto, Nishimura Kazuko
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan.
J Clin Microbiol. 2003 Feb;41(2):873-6. doi: 10.1128/JCM.41.2.873-876.2003.
An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus FONSECAEA:
设计了一种基于核糖体DNA内部转录间隔区的寡核苷酸引物组用于PCR,该引物组仅对来自丰色卡氏菌属物种的DNA产生扩增子。对于所有检测的佩德罗丰色卡氏菌和紧密丰色卡氏菌菌株,该引物组产生了一个333 bp的扩增子,但相关的暗色丝孢菌和致病性酵母未产生扩增子。使用该引物组进行PCR被认为是快速鉴定丰色卡氏菌属的一种有用方法。