Mekkes J R, Le Poole I C, Das P K, Bos J D, Westerhof W
Department of Dermatology, Academisch Medisch Centrum, University of Amsterdam, Amsterdam, The Netherlands.
Wound Repair Regen. 1998 Jan-Feb;6(1):50-7. doi: 10.1046/j.1524-475x.1998.60108.x.
Wound healing can be accelerated by removing necrotic tissue. Various methods of wound debridement have been developed, including enzymatic debridement. Recently potent proteolytic enzymes were isolated from the intestine of Euphausia superba (Antarctic krill) that might be useful for degrading necrotic tissue. The purpose of this study was to evaluate the debriding properties of krill enzymes, using a specially designed animal model and a computerized analysis system. In 10 female domestic pigs, each weighing 20 kg, 6 artificial ulcers were made on each animal's back using electrokeratome, followed by application of trichloracetic acid. Ulcers were treated twice daily for 7 days with either krill enzymes at different concentrations or with saline. Reduction of necrotic tissue was measured daily using computerized wound analysis. Histological examination included the determination of bromodeoxyuridine incorporation in order to detect cell proliferation as well as routine stains. The debriding effect of krill enzymes at a concentration of >/= 3.0 casein units per ml was significantly better than saline control treatment (p < 0.05). The effect was dose dependent, and granulation tissue formation was enhanced. In conclusion, krill enzymes are effective in wound debridement, as measured in this animal model.
去除坏死组织可加速伤口愈合。人们已开发出多种伤口清创方法，包括酶促清创法。最近，从南极磷虾的肠道中分离出了强效蛋白水解酶，这些酶可能有助于降解坏死组织。本研究的目的是使用专门设计的动物模型和计算机分析系统，评估磷虾酶的清创特性。在10头体重均为20千克的雌性家猪身上，用角膜刀在每头动物的背部制造6处人工溃疡，然后涂抹三氯乙酸。溃疡每天用不同浓度的磷虾酶或生理盐水治疗两次，持续7天。每天使用计算机化伤口分析测量坏死组织的减少情况。组织学检查包括测定溴脱氧尿苷掺入情况以检测细胞增殖以及常规染色。每毫升浓度≥3.0酪蛋白单位的磷虾酶的清创效果明显优于生理盐水对照治疗（p<0.05）。效果呈剂量依赖性，且肉芽组织形成得到增强。总之，在该动物模型中测定，磷虾酶在伤口清创方面有效。
