Applications of boronate derivatives in the study of ceramides by gas-liquid chromatography-mass spectrometry.

Methaneboronate derivatives of ceramides possess excellent gas-liquid chromatographic properties and give informative mass spectra. Molecular ions, present at high abundance where an unsaturated acyl substituent is present, are accompanied in the electron impact mass spectra by fragment ions which denote the acyl group and long-chain base. In the gas-liquid chromatographic-mass spectrometric analyses of natural ceramides, as methaneboronate derivatives, the properties of open-tubular columns are exploited to give good separations in moderate analysis times. An application is made to the analysis of ceramides derived from sphingomyelin of human arterial tissue.