Uyar F A, Imeryüz N, Saruhan-Direskeneli G, Ceken H, Ozdoğan O, Sahin S, Tözün N
Department of Physiology, Istanbul Medical Faculty, University of Istanbul, Turkey.
Eur J Immunogenet. 1998 Aug;25(4):293-6. doi: 10.1046/j.1365-2370.1998.00104.x.
Recently described distinct associations of HLA class II genes with ulcerative colitis (UC) suggest a genetic heterogeneity for disease susceptibility. In this study, HLA-DRB alleles of UC patients (n = 59) from Turkey were investigated and compared with healthy controls (n = 244). Using molecular genotyping by polymerase chain reaction (PCR) and sequence-specific oligonucleotide hybridization, we have shown a positive association of UC patients with the HLA-DRB11502 allele (10/59 vs. 16/244; P = 0.02; OR: 2.9) and a negative association with the DRB113 allele (7/59 vs. 64/244; P = 0.03; OR: 0.38) compared to controls. HLA-DRB10701 was significantly increased in perinuclear antineutrophil cytoplasmic antibody (pANCA)-positive UC patients compared to pANCA-negative patients (8/32 vs. 0/27; P = 0.005), whereas DRB11502 was observed more frequently in pANCA-negative patients (8/27 vs. 2/32; P = 0.03). These results extended the reported positive association of DRB1*1502 with UC to another population and supported the genetic susceptibility associated with HLA genes for disease development.
最近描述的HLA II类基因与溃疡性结肠炎（UC）的不同关联表明疾病易感性存在遗传异质性。在本研究中，对来自土耳其的UC患者（n = 59）的HLA - DRB等位基因进行了调查，并与健康对照（n = 244）进行了比较。使用聚合酶链反应（PCR）和序列特异性寡核苷酸杂交进行分子基因分型，我们发现UC患者与HLA - DRB11502等位基因呈正相关（10/59 vs. 16/244；P = 0.02；OR：2.9），与DRB113等位基因呈负相关（7/59 vs. 64/244；P = 0.03；OR：0.38）。与抗中性粒细胞胞浆抗体（pANCA）阴性的UC患者相比，pANCA阳性的UC患者中HLA - DRB10701显著增加（8/32 vs. 0/27；P = 0.005），而DRB11502在pANCA阴性患者中更常见（8/27 vs. 2/32；P = 0.03）。这些结果将报道的DRB1*1502与UC的正相关扩展到了另一人群，并支持了与HLA基因相关的疾病发生遗传易感性。
