Vanheusden A, Nusgens B, Goffinet G, Zahedi S, Lapière C M, Rompen E
Institute of Dentistry, University of Liège, Belgium.
J Periodontal Res. 1998 Aug;33(6):377-85. doi: 10.1111/j.1600-0765.1998.tb02213.x.
Although the influence of tetracyclines on periodontal connective tissue cells has been the topic of many in vitro and in vivo studies, data regarding their effects on gingival epithelial cells are scarce. The present in vitro study was designed to examine the influence of minocycline, a semi-synthetic analog of tetracycline, on human gingival keratinocyte (HGK) attachment and migration. Attachment tests were performed with HGK prelabeled by tritiated amino-acids. Increasing concentrations of minocycline (10, 50, 100 micrograms/ml) in the medium produced no significant modification of cell adhesion kinetics compared to control conditions, except for 100 micrograms/ml which statistically significantly (p < 0.05) reduced the number of attached cells beyond 6 h. A 24-h cell preincubation in 10 micrograms/ml of minocycline did not alter the kinetics of HGK attachment. Scanning electron microscopic observations of attached HGK showed that the presence of 10 micrograms/ml of minocycline in the "attachment medium" induced the production of multiple filopodial extensions. Migration tests in Boyden chambers for 40 h demonstrated that HGK preincubation for 24 h in a 10 micrograms/ml minocycline-HCl solution increased significantly (p < 0.005) cell migration towards a gradient of fetal calf serum. The presence of 10 micrograms/ml of minocycline in contact with the keratinocytes in the upper compartment of the migration chambers also produced a significant (p < 0.005) result. In contrast, the presence of minocycline in the lower compartments did not produce any chemoattractive effect. Within the limits of their significance, these results suggest that, at concentrations not beyond 50 micrograms/ml, minocycline could fasten the periodontal wound coverage by epithelial cells and allow the normal reformation of a junctional epithelium.
尽管四环素对牙周结缔组织细胞的影响已成为许多体外和体内研究的主题，但关于其对牙龈上皮细胞作用的数据却很少。本体外研究旨在检测米诺环素（一种四环素的半合成类似物）对人牙龈角质形成细胞（HGK）黏附和迁移的影响。黏附试验使用经氚标记氨基酸预标记的HGK进行。与对照条件相比，培养基中米诺环素浓度增加（10、50、100微克/毫升）对细胞黏附动力学没有显著改变，但100微克/毫升时，6小时后附着细胞数量在统计学上显著减少（p<0.05）。在10微克/毫升米诺环素中进行24小时细胞预孵育并未改变HGK的黏附动力学。对附着的HGK进行扫描电子显微镜观察显示，“黏附培养基”中存在10微克/毫升米诺环素会诱导多个丝状伪足延伸的产生。在Boyden小室中进行40小时的迁移试验表明，在10微克/毫升盐酸米诺环素溶液中预孵育24小时的HGK向胎牛血清梯度的细胞迁移显著增加（p<0.005）。迁移小室上室中与角质形成细胞接触的10微克/毫升米诺环素也产生了显著结果（p<0.005）。相比之下，下室中米诺环素的存在没有产生任何趋化作用。在其显著性范围内，这些结果表明，在浓度不超过50微克/毫升时，米诺环素可以加快上皮细胞对牙周伤口的覆盖，并使结合上皮正常重塑。
