Shibutani T, Imai K, Kanazawa A, Iwayama Y
Department of Periodontology, Asahi Universuty, School of Dentistry, Gifu, Japan.
J Periodontal Res. 1998 Jul;33(5):265-73. doi: 10.1111/j.1600-0765.1998.tb02199.x.
This study was designed to demonstrate, by use of biotin-labeled hyaluronic acid binding protein (HABP) and an avidin-enzyme system, the localization of hyaluronan (HA) in periodontal tissue of beagle dogs during experimentally induced periodontitis. Experimental periodontitis was induced in the dogs by ligation of the gingival sulcus. Experimental tissue was collected at 0, 3, 7 and 21 days after ligation. HA was revealed by strong staining in the intercellular space around epithelial cells and periodontal ligament, and by light staining in the gingival connective tissue. According to the progression of periodontal tissue breakdown, HA was detected in a small number of leukocytes and monocytes, on the surface of osteoclasts, the surface of alveolar bone, thickened endothelium and in epithelial cells related to rete peg formation. Streptomyces hyaluronidase-treated specimens gave negative staining. This study suggests that HA may be associated with the inflammatory reaction in experimental periodontitis tissue.
本研究旨在通过使用生物素标记的透明质酸结合蛋白（HABP）和抗生物素蛋白-酶系统，证明实验性诱导牙周炎期间比格犬牙周组织中透明质酸（HA）的定位。通过结扎牙龈沟在犬中诱导实验性牙周炎。在结扎后0、3、7和21天收集实验组织。HA在上皮细胞和牙周膜周围的细胞间隙中呈强染色，在牙龈结缔组织中呈淡染色。根据牙周组织破坏的进展情况，在少数白细胞和单核细胞、破骨细胞表面、牙槽骨表面、增厚的内皮以及与 rete peg 形成相关的上皮细胞中检测到HA。经链霉菌透明质酸酶处理的标本呈阴性染色。本研究表明，HA可能与实验性牙周炎组织中的炎症反应有关。
