Department of Clinic and Molecular Sciences-Histology, Polytechnic University of Marche, Via Tronto 10/a-60126, Torrette, Ancona, Italy.
Pathologic Anatomy and Histopathology Division, Department of Biomedical Sciences and Public Health, Polytechnic University of the Marche, Ancona, Italy.
Inflammation. 2019 Apr;42(2):682-689. doi: 10.1007/s10753-018-0926-0.
Several biologic processes affect the supporting peri-implant tissue leading to implant failure and complications, mainly referred to inflammation that is still poorly investigated in the peri-implant soft tissues. Our aim was to investigate in peri-implant healthy mucosa, peri-implant mucositis, and peri-implantitis the expression of some angiogenesis markers highly associated with inflammation, and evaluate its relationships with age, smoking, peri-implant pocket depth (PPD), and body max index (BMI). Moreover, we wanted to study the impact of these clinical parameters in the disease pathogenesis. Forty-eight total patients were recruited. Sixteen had at least one successfully osteointegrated dental implant (group A) and 32 had at least one osseointegrated implant in need of a peri-implant treatment for inflammatory/infectiveous reasons: precisely 16 for mucositis (group B) and 16 for peri-implantitis (group C). VEGF, CD34, and CD44 immunohistochemical expression was evaluated in the interproximal biopsies of marginal peri-implant tissue and correlated with the clinical parameters. A significant difference between groups in mean PPD was found, while the distribution by age, gender, smoking, and BMI resulted similar. Group C had significantly higher levels of VEGF, CD34, and CD44 expression compared to the other groups. VEGF, CD34, CD44, and peri-implant pocket depth were all positively correlated. Our study revealed that peri-implantitis is a condition characterized by unique and distinctive features. Our results supported that PPD has a great impact on the peri-implantitis and it is closely related to the inflammation marker expression. The identification of specific biomarkers might help in choosing distinct treatment approaches for target individuals.
几种生物学过程会影响支持种植体周围组织，导致种植体失败和并发症，主要是炎症，而种植体周围软组织中的炎症仍研究不足。我们的目的是研究种植体健康黏膜、种植体周围黏膜炎和种植体周围炎中与炎症高度相关的一些血管生成标志物，并评估其与年龄、吸烟、种植体周袋深度（PPD）和体重指数（BMI）的关系。此外，我们还希望研究这些临床参数在疾病发病机制中的作用。共招募了 48 名患者。16 名患者至少有一颗成功骨整合的牙种植体（A 组），32 名患者至少有一颗需要进行种植体周围治疗的骨整合种植体，原因是炎症/感染：16 名患者为黏膜炎（B 组），16 名患者为种植体周围炎（C 组）。在边缘种植体周围组织的邻间活检中评估了 VEGF、CD34 和 CD44 的免疫组织化学表达，并与临床参数相关联。发现组间 PPD 的平均值存在显著差异，而年龄、性别、吸烟和 BMI 的分布相似。与其他组相比，C 组的 VEGF、CD34 和 CD44 表达水平显著升高。VEGF、CD34、CD44 和种植体周袋深度均呈正相关。我们的研究表明，种植体周围炎是一种具有独特特征的疾病。我们的研究结果表明，PPD 对种植体周围炎有很大影响，并且与炎症标志物的表达密切相关。特定生物标志物的鉴定可能有助于为特定个体选择不同的治疗方法。
